Development of a Nucleic Acid Lateral Flow Immunoassay for the Detection of Human Polyomavirus BK

The BK virus (BKV) is an emerging pathogen in immunocompromised individuals and widespread in the human population. Polymerase chain reaction is a simple and highly sensitive method for detecting BKV, but it is time consuming and requires expensive instruments and expert judgment. The lateral flow a...

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Autores principales: Huang, Yi-Huei, Yu, Kuan-Yi, Huang, Shou-Ping, Chuang, Hui-Wen, Lin, Wen-Zhi, Cherng, Juin-Hong, Hung, Yao-Wen, Yeh, Ming-Kung, Hong, Po-Da, Liu, Cheng-Che
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345645/
https://www.ncbi.nlm.nih.gov/pubmed/32545649
http://dx.doi.org/10.3390/diagnostics10060403
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author Huang, Yi-Huei
Yu, Kuan-Yi
Huang, Shou-Ping
Chuang, Hui-Wen
Lin, Wen-Zhi
Cherng, Juin-Hong
Hung, Yao-Wen
Yeh, Ming-Kung
Hong, Po-Da
Liu, Cheng-Che
author_facet Huang, Yi-Huei
Yu, Kuan-Yi
Huang, Shou-Ping
Chuang, Hui-Wen
Lin, Wen-Zhi
Cherng, Juin-Hong
Hung, Yao-Wen
Yeh, Ming-Kung
Hong, Po-Da
Liu, Cheng-Che
author_sort Huang, Yi-Huei
collection PubMed
description The BK virus (BKV) is an emerging pathogen in immunocompromised individuals and widespread in the human population. Polymerase chain reaction is a simple and highly sensitive method for detecting BKV, but it is time consuming and requires expensive instruments and expert judgment. The lateral flow assay, a rapid, low-cost, minimal-labor, and easy-to-use diagnostic method, was successfully applied for pathogen detection. In this study, we used oligonucleotide probes to develop a simple and rapid sandwich-type lateral flow immunoassay for detecting BKV DNA within 45 minutes. The detection limit for the synthetic single-stranded DNA was 5 nM. The specificity study showed no cross-reactivity with other polyomaviruses, such as JC virus and simian virus 40. For the Escherichia coli containing BKV plasmid cultured samples, the sensitivity was determined to be 10(7) copies/mL. The approach offers great potential for BKV detection of various target analytes in point-of-care settings.
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spelling pubmed-73456452020-07-09 Development of a Nucleic Acid Lateral Flow Immunoassay for the Detection of Human Polyomavirus BK Huang, Yi-Huei Yu, Kuan-Yi Huang, Shou-Ping Chuang, Hui-Wen Lin, Wen-Zhi Cherng, Juin-Hong Hung, Yao-Wen Yeh, Ming-Kung Hong, Po-Da Liu, Cheng-Che Diagnostics (Basel) Article The BK virus (BKV) is an emerging pathogen in immunocompromised individuals and widespread in the human population. Polymerase chain reaction is a simple and highly sensitive method for detecting BKV, but it is time consuming and requires expensive instruments and expert judgment. The lateral flow assay, a rapid, low-cost, minimal-labor, and easy-to-use diagnostic method, was successfully applied for pathogen detection. In this study, we used oligonucleotide probes to develop a simple and rapid sandwich-type lateral flow immunoassay for detecting BKV DNA within 45 minutes. The detection limit for the synthetic single-stranded DNA was 5 nM. The specificity study showed no cross-reactivity with other polyomaviruses, such as JC virus and simian virus 40. For the Escherichia coli containing BKV plasmid cultured samples, the sensitivity was determined to be 10(7) copies/mL. The approach offers great potential for BKV detection of various target analytes in point-of-care settings. MDPI 2020-06-12 /pmc/articles/PMC7345645/ /pubmed/32545649 http://dx.doi.org/10.3390/diagnostics10060403 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Huang, Yi-Huei
Yu, Kuan-Yi
Huang, Shou-Ping
Chuang, Hui-Wen
Lin, Wen-Zhi
Cherng, Juin-Hong
Hung, Yao-Wen
Yeh, Ming-Kung
Hong, Po-Da
Liu, Cheng-Che
Development of a Nucleic Acid Lateral Flow Immunoassay for the Detection of Human Polyomavirus BK
title Development of a Nucleic Acid Lateral Flow Immunoassay for the Detection of Human Polyomavirus BK
title_full Development of a Nucleic Acid Lateral Flow Immunoassay for the Detection of Human Polyomavirus BK
title_fullStr Development of a Nucleic Acid Lateral Flow Immunoassay for the Detection of Human Polyomavirus BK
title_full_unstemmed Development of a Nucleic Acid Lateral Flow Immunoassay for the Detection of Human Polyomavirus BK
title_short Development of a Nucleic Acid Lateral Flow Immunoassay for the Detection of Human Polyomavirus BK
title_sort development of a nucleic acid lateral flow immunoassay for the detection of human polyomavirus bk
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345645/
https://www.ncbi.nlm.nih.gov/pubmed/32545649
http://dx.doi.org/10.3390/diagnostics10060403
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