Critique of the pairwise method for estimating qPCR amplification efficiency: beware of correlated data!

BACKGROUND: A recently proposed method for estimating qPCR amplification efficiency E analyzes fluorescence intensity ratios from pairs of points deemed to lie in the exponential growth region on the amplification curves for all reactions in a dilution series. This method suffers from a serious prob...

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Detalles Bibliográficos
Autor principal: Tellinghuisen, Joel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7346608/
https://www.ncbi.nlm.nih.gov/pubmed/32640980
http://dx.doi.org/10.1186/s12859-020-03604-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7346608/
https://www.ncbi.nlm.nih.gov/pubmed/32640980
http://dx.doi.org/10.1186/s12859-020-03604-4

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