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Visualization of cytoplasmic organelles via in-resin CLEM using an osmium-resistant far-red protein

Post-fixation with osmium tetroxide staining and the embedding of Epon are robust and essential treatments that are used to preserve and visualize intracellular membranous structures during electron microscopic analyses. These treatments, however, can significantly diminish the fluorescent intensity...

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Detalles Bibliográficos
Autores principales: Tanida, Isei, Kakuta, Soichiro, Oliva Trejo, Juan Alejandro, Uchiyama, Yasuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347593/
https://www.ncbi.nlm.nih.gov/pubmed/32647231
http://dx.doi.org/10.1038/s41598-020-68191-z