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Visualization of cytoplasmic organelles via in-resin CLEM using an osmium-resistant far-red protein
Post-fixation with osmium tetroxide staining and the embedding of Epon are robust and essential treatments that are used to preserve and visualize intracellular membranous structures during electron microscopic analyses. These treatments, however, can significantly diminish the fluorescent intensity...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347593/ https://www.ncbi.nlm.nih.gov/pubmed/32647231 http://dx.doi.org/10.1038/s41598-020-68191-z |