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Establishment and validation of highly accurate formalin-fixed paraffin-embedded quantitative proteomics by heat-compatible pressure cycling technology using phase-transfer surfactant and SWATH-MS

The purpose of this study was to establish a quantitative proteomic method able to accurately quantify pathological changes in the protein expression levels of not only non-membrane proteins, but also membrane proteins, using formalin-fixed paraffin-embedded (FFPE) samples. Protein extraction from F...

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Detalles Bibliográficos
Autores principales: Uchida, Yasuo, Sasaki, Hayate, Terasaki, Tetsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347883/
https://www.ncbi.nlm.nih.gov/pubmed/32647189
http://dx.doi.org/10.1038/s41598-020-68245-2