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凝血因子Ⅸ野生型pIRES2-ZsGreen1真核表达载体的构建及其在HEK-293细胞的表达
OBJECTIVE: To construct pIRES2-ZsGreen1/F Ⅸ expression vector, using the pcDNA/FⅨ plasmid containing FⅨ cDNA as template, and express in HEK-293 cells. METHODS: The total ORF of F Ⅸ gene was amplified from pcDNA/F Ⅸ plasmid, then the amplified fragment was clonded into the pIRES2-ZsGreen1 vector usi...
| Formato: | Online Artículo Texto |
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| Lenguaje: | English |
| Publicado: |
Editorial office of Chinese Journal of Hematology
2016
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7348507/ https://www.ncbi.nlm.nih.gov/pubmed/27995883 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2016.11.009 |