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Rapid Evaluation of CRISPR Guides and Donors for Engineering Mice

Although the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/ CRISPR associated protein 9 (Cas9) technique has dramatically lowered the cost and increased the speed of generating genetically engineered mice, success depends on using guide RNAs and donor DNAs which direct efficient...

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Detalles Bibliográficos
Autores principales: McBeath, Elena, Parker-Thornburg, Jan, Fujii, Yuka, Aryal, Neeraj, Smith, Chad, Hofmann, Marie-Claude, Abe, Jun-ichi, Fujiwara, Keigi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349695/
https://www.ncbi.nlm.nih.gov/pubmed/32521708
http://dx.doi.org/10.3390/genes11060628