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Rapid Evaluation of CRISPR Guides and Donors for Engineering Mice
Although the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/ CRISPR associated protein 9 (Cas9) technique has dramatically lowered the cost and increased the speed of generating genetically engineered mice, success depends on using guide RNAs and donor DNAs which direct efficient...
Autores principales: | McBeath, Elena, Parker-Thornburg, Jan, Fujii, Yuka, Aryal, Neeraj, Smith, Chad, Hofmann, Marie-Claude, Abe, Jun-ichi, Fujiwara, Keigi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349695/ https://www.ncbi.nlm.nih.gov/pubmed/32521708 http://dx.doi.org/10.3390/genes11060628 |
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