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A Sample Preparation Protocol for High Throughput Immunofluorescence of Suspension Cells on an Adherent Surface

Imaging is a powerful approach for studying protein expression and has the advantage over other methodologies in providing spatial information in situ at single cell level. Using immunofluorescence and confocal microscopy, detailed information of subcellular distribution of proteins can be obtained....

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Detalles Bibliográficos
Autores principales: Bäckström, Anna, Kugel, Laura, Gnann, Christian, Xu, Hao, Aslan, Joseph E., Lundberg, Emma, Stadler, Charlotte
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350080/
https://www.ncbi.nlm.nih.gov/pubmed/32564662
http://dx.doi.org/10.1369/0022155420935403