One-step heating strategy for efficient solubilization of recombinant spider silk protein from inclusion bodies

BACKGROUND: Spider silk is a proteinaceous fiber with remarkable mechanical properties spun from spider silk proteins (spidroins). Engineering spidroins have been successfully produced in a variety of heterologous hosts and the most widely used expression system is Escherichia coli (E. coli). So far...

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Detalles Bibliográficos
Autores principales: Cai, Hui, Chen, Gefei, Yu, Hairui, Tang, Ying, Xiong, Sidong, Qi, Xingmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350728/
https://www.ncbi.nlm.nih.gov/pubmed/32650749
http://dx.doi.org/10.1186/s12896-020-00630-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350728/
https://www.ncbi.nlm.nih.gov/pubmed/32650749
http://dx.doi.org/10.1186/s12896-020-00630-1

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