Cargando…

One-step heating strategy for efficient solubilization of recombinant spider silk protein from inclusion bodies

BACKGROUND: Spider silk is a proteinaceous fiber with remarkable mechanical properties spun from spider silk proteins (spidroins). Engineering spidroins have been successfully produced in a variety of heterologous hosts and the most widely used expression system is Escherichia coli (E. coli). So far...

Descripción completa

Detalles Bibliográficos
Autores principales:	Cai, Hui, Chen, Gefei, Yu, Hairui, Tang, Ying, Xiong, Sidong, Qi, Xingmei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2020
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350728/ https://www.ncbi.nlm.nih.gov/pubmed/32650749 http://dx.doi.org/10.1186/s12896-020-00630-1

Ejemplares similares

Importance of Heat and Pressure for Solubilization of Recombinant Spider Silk Proteins in Aqueous Solution
por: Jones, Justin A., et al.
Publicado: (2016)

A single freeze-thawing cycle for highly efficient solubilization of inclusion body proteins and its refolding into bioactive form
por: Qi, Xingmei, et al.
Publicado: (2015)

Recombinant Spider Silk: Promises and Bottlenecks
por: Ramezaniaghdam, Maryam, et al.
Publicado: (2022)

Recombinant DNA production of spider silk proteins
por: Tokareva, Olena, et al.
Publicado: (2013)

Self-Healing of Recombinant Spider Silk Gel and Coating
por: Wu, Shin-Da, et al.
Publicado: (2023)

Customized Flagelliform Spidroins Form Spider Silk-like Fibers at pH 8.0 with Outstanding Tensile Strength
por: Li, Xue, et al.
Publicado: (2021)

Site‐Specific Functionalization of Recombinant Spider Silk Janus Fibers
por: Lang, Gregor, et al.
Publicado: (2022)

Two-step self-assembly of a spider silk molecular clamp
por: Rat, Charlotte, et al.
Publicado: (2018)

Spider silks: recombinant synthesis, assembly, spinning, and engineering of synthetic proteins
por: Scheibel, Thomas
Publicado: (2004)

Tissue Response to Subcutaneously Implanted Recombinant Spider Silk: An in Vivo Study
por: Fredriksson, Camilla, et al.
Publicado: (2009)

Recombinant spider silk coatings functionalized with enzymes targeting bacteria and biofilms
por: Seijsing, Fredrik, et al.
Publicado: (2020)

Impacts of Blended Bombyx mori Silk Fibroin and Recombinant Spider Silk Fibroin Hydrogels on Cell Growth
por: Laomeephol, Chavee, et al.
Publicado: (2021)

Spider silk gut: Development and characterization of a novel strong spider silk fiber
por: Jiang, Ping, et al.
Publicado: (2014)

Efficient protein production inspired by how spiders make silk
por: Kronqvist, Nina, et al.
Publicado: (2017)

How Spiders Spin Silk
por: Meadows, Robin
Publicado: (2014)

Silk Spinning in Silkworms and Spiders
por: Andersson, Marlene, et al.
Publicado: (2016)

The myth of antibiotic spider silk
por: Fruergaard, Simon, et al.
Publicado: (2021)

Complexity of Spider Dragline Silk
por: Malay, Ali D., et al.
Publicado: (2022)

Effect of buffer additives on solubilization and refolding of reteplase inclusion bodies
por: Esmaili, Iman, et al.
Publicado: (2018)

State-of-the-art and novel approaches to mild solubilization of inclusion bodies
por: Klausser, Robert, et al.
Publicado: (2023)

Infection Responsive Smart Delivery of Antibiotics Using Recombinant Spider Silk Nanospheres
por: Mulinti, Pranothi, et al.
Publicado: (2021)

Solubilization and purification of recombinant modified C-reactive protein from inclusion bodies using reversible anhydride modification
por: Potempa, Lawrence A., et al.
Publicado: (2015)

Structural characterization of recombinant streptokinase following recovery from inclusion bodies using different chemical solubilization treatments
por: Babaei Sheli, Khadijeh, et al.
Publicado: (2018)

Spontaneous C-cleavage of a truncated intein as fusion tag to produce tag-free VP1 inclusion body nanoparticle vaccine against CVB3-induced viral myocarditis by the oral route
por: Qi, Xingmei, et al.
Publicado: (2019)

Mechanical and Physical Properties of Recombinant Spider Silk Films Using Organic and Aqueous Solvents
por: Tucker, Chauncey L., et al.
Publicado: (2014)

Recombinant Minimalist Spider Wrapping Silk Proteins Capable of Native-Like Fiber Formation
por: Xu, Lingling, et al.
Publicado: (2012)

Air Filter Devices Including Nonwoven Meshes of Electrospun Recombinant Spider Silk Proteins
por: Lang, Gregor, et al.
Publicado: (2013)

Recombinant Spider Silk Gels Derived from Aqueous–Organic Solvents as Depots for Drugs
por: Neubauer, Vanessa J., et al.
Publicado: (2021)

Flow Simulation and Gradient Printing of Fluorapatite- and Cell-Loaded Recombinant Spider Silk Hydrogels
por: Neubauer, Vanessa J., et al.
Publicado: (2022)

Recombinant Spider Silk Fiber with High Dimensional Stability in Water and Its NMR Characterization
por: Asakura, Tetsuo, et al.
Publicado: (2022)

Biocompatible Optical Fibers Made of Regenerated Cellulose and Recombinant Cellulose-Binding Spider Silk
por: Reimer, Martin, et al.
Publicado: (2023)

Hybrid Spider Silk with Inorganic Nanomaterials
por: Kiseleva, Aleksandra P., et al.
Publicado: (2020)

Spidroins and Silk Fibers of Aquatic Spiders
por: Correa-Garhwal, Sandra M., et al.
Publicado: (2019)

Spider Silk for Tissue Engineering Applications
por: Salehi, Sahar, et al.
Publicado: (2020)

Spider Silk‐Inspired Artificial Fibers
por: Li, Jiatian, et al.
Publicado: (2021)

The method of purifying bioengineered spider silk determines the silk sphere properties
por: Jastrzebska, Katarzyna, et al.
Publicado: (2016)

Tailor-made spider-eggcase-silk spheres for efficient lysosomal drug delivery
por: Chen, Jianming, et al.
Publicado: (2018)

Recombinant spider silk from aqueous solutions via a bio-inspired microfluidic chip
por: Peng, Qingfa, et al.
Publicado: (2016)

Studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing
por: Zhao, Liang, et al.
Publicado: (2017)

Presence of β-Turn Structure in Recombinant Spider Silk Dissolved in Formic Acid Revealed with NMR
por: Suzuki, Yu, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_6o6ep5fku5g7r31jjj779up65g