Inflammation changes the expression of neuropeptide Y receptors in the pig myometrium and their role in the uterine contractility

In the experiment the influence of inflammation on neuropeptide Y (NPY) receptor subtype 1 (Y1Rs) and 2 (Y2Rs) expression pattern in the gilt myometrium and NPY effect alone or with Y1Rs and Y2Rs antagonists on contractility of an inflamed uterus were recognized. On Day 3 of the estrous cycle, eithe...

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Autores principales: Jana, Barbara, Całka, Jarosław, Palus, Katarzyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351157/
https://www.ncbi.nlm.nih.gov/pubmed/32649683
http://dx.doi.org/10.1371/journal.pone.0236044
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author Jana, Barbara
Całka, Jarosław
Palus, Katarzyna
author_facet Jana, Barbara
Całka, Jarosław
Palus, Katarzyna
author_sort Jana, Barbara
collection PubMed
description In the experiment the influence of inflammation on neuropeptide Y (NPY) receptor subtype 1 (Y1Rs) and 2 (Y2Rs) expression pattern in the gilt myometrium and NPY effect alone or with Y1Rs and Y2Rs antagonists on contractility of an inflamed uterus were recognized. On Day 3 of the estrous cycle, either E.coli suspension (E.coli group) or saline (SAL group) were administered into uterine horns. In the control gilts (CON group), only laparotomy was carried out. Eight days later, E.coli treatment evoked severe acute endometritis, significantly reduced Y1Rs mRNA and protein expression and increased Y2Rs protein expression in myometrium in relation to the CON and SAL groups. Compared to period before NPY application, NPY (10(−7) M) significantly reduced amplitude in myometrium and endometrium/myometrium and frequency in myometrium of the CON and SAL groups and amplitude in endometrium/myometrium and frequency in strips of the E.coli group. In this group, after using NPY (10(−7) M), the amplitude rose significantly in both kinds of strips, while frequency fell in endometrium/myometrium in relation to the SAL group. In the CON and SAL groups, NPY (10(−7) M) with Y1Rs antagonist significantly increased the amplitude in endometrium/myometrium, while with Y2Rs antagonist caused a rise in myometrium. In the E.coli group after using Y1Rs or Y2Rs antagonist and NPY (10(−7) M) amplitude did not significantly change in endometrium/myometrium, and this parameter was significantly lower after using the NPY and Y1Rs antagonist than in other groups. Y1Rs antagonist and NPY (10(−8), 10(−7) M) significantly increased frequency in endometrium/myometrium of the CON and SAL groups. In the E.coli group, Y1Rs or Y2Rs antagonists and NPY (10(−7) M) significantly increased frequency in myometrium and it was significantly higher than in the CON group. Inflammation changes Y1Rs and Y2Rs expression in myometrium of the inflamed pig uterus and NPY reduces this organ contractility by Y1Rs and Y2Rs.
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spelling pubmed-73511572020-07-20 Inflammation changes the expression of neuropeptide Y receptors in the pig myometrium and their role in the uterine contractility Jana, Barbara Całka, Jarosław Palus, Katarzyna PLoS One Research Article In the experiment the influence of inflammation on neuropeptide Y (NPY) receptor subtype 1 (Y1Rs) and 2 (Y2Rs) expression pattern in the gilt myometrium and NPY effect alone or with Y1Rs and Y2Rs antagonists on contractility of an inflamed uterus were recognized. On Day 3 of the estrous cycle, either E.coli suspension (E.coli group) or saline (SAL group) were administered into uterine horns. In the control gilts (CON group), only laparotomy was carried out. Eight days later, E.coli treatment evoked severe acute endometritis, significantly reduced Y1Rs mRNA and protein expression and increased Y2Rs protein expression in myometrium in relation to the CON and SAL groups. Compared to period before NPY application, NPY (10(−7) M) significantly reduced amplitude in myometrium and endometrium/myometrium and frequency in myometrium of the CON and SAL groups and amplitude in endometrium/myometrium and frequency in strips of the E.coli group. In this group, after using NPY (10(−7) M), the amplitude rose significantly in both kinds of strips, while frequency fell in endometrium/myometrium in relation to the SAL group. In the CON and SAL groups, NPY (10(−7) M) with Y1Rs antagonist significantly increased the amplitude in endometrium/myometrium, while with Y2Rs antagonist caused a rise in myometrium. In the E.coli group after using Y1Rs or Y2Rs antagonist and NPY (10(−7) M) amplitude did not significantly change in endometrium/myometrium, and this parameter was significantly lower after using the NPY and Y1Rs antagonist than in other groups. Y1Rs antagonist and NPY (10(−8), 10(−7) M) significantly increased frequency in endometrium/myometrium of the CON and SAL groups. In the E.coli group, Y1Rs or Y2Rs antagonists and NPY (10(−7) M) significantly increased frequency in myometrium and it was significantly higher than in the CON group. Inflammation changes Y1Rs and Y2Rs expression in myometrium of the inflamed pig uterus and NPY reduces this organ contractility by Y1Rs and Y2Rs. Public Library of Science 2020-07-10 /pmc/articles/PMC7351157/ /pubmed/32649683 http://dx.doi.org/10.1371/journal.pone.0236044 Text en © 2020 Jana et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Jana, Barbara
Całka, Jarosław
Palus, Katarzyna
Inflammation changes the expression of neuropeptide Y receptors in the pig myometrium and their role in the uterine contractility
title Inflammation changes the expression of neuropeptide Y receptors in the pig myometrium and their role in the uterine contractility
title_full Inflammation changes the expression of neuropeptide Y receptors in the pig myometrium and their role in the uterine contractility
title_fullStr Inflammation changes the expression of neuropeptide Y receptors in the pig myometrium and their role in the uterine contractility
title_full_unstemmed Inflammation changes the expression of neuropeptide Y receptors in the pig myometrium and their role in the uterine contractility
title_short Inflammation changes the expression of neuropeptide Y receptors in the pig myometrium and their role in the uterine contractility
title_sort inflammation changes the expression of neuropeptide y receptors in the pig myometrium and their role in the uterine contractility
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351157/
https://www.ncbi.nlm.nih.gov/pubmed/32649683
http://dx.doi.org/10.1371/journal.pone.0236044
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