Isolation and diversity of sediment bacteria in the hypersaline aiding lake, China

Halophiles are relatively unexplored as potential sources of novel species. However, little is known about the culturable bacterial diversity thrive in hypersaline lakes. In this work, a total of 343 bacteria from sediment samples of Aiding Lake, China, were isolated using nine different media suppl...

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Autores principales: Guan, Tong-Wei, Lin, Yi-Jin, Ou, Meng-Ying, Chen, Ke-Bao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351256/
https://www.ncbi.nlm.nih.gov/pubmed/32649724
http://dx.doi.org/10.1371/journal.pone.0236006
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author Guan, Tong-Wei
Lin, Yi-Jin
Ou, Meng-Ying
Chen, Ke-Bao
author_facet Guan, Tong-Wei
Lin, Yi-Jin
Ou, Meng-Ying
Chen, Ke-Bao
author_sort Guan, Tong-Wei
collection PubMed
description Halophiles are relatively unexplored as potential sources of novel species. However, little is known about the culturable bacterial diversity thrive in hypersaline lakes. In this work, a total of 343 bacteria from sediment samples of Aiding Lake, China, were isolated using nine different media supplemented with 5% or 15% (w/v) NaCl. The number of species and genera of bacteria recovered from the different media varied, indicating the need to optimize the isolation conditions. The results showed an unexpected level of bacterial diversity, with four phyla (Actinobacteria, Firmicutes, Proteobacteria, and Rhodothermaeota), fourteen orders (Actinopolysporales, Alteromonadales, Bacillales, Balneolales, Chromatiales, Glycomycetales, Jiangellales, Micrococcales, Micromonosporales, Oceanospirillales, Pseudonocardiales, Rhizobiales, Streptomycetales, and Streptosporangiales), including 17 families, 43 genera (including two novel genera), and 71 species (including four novel species). The predominant phyla included Actinobacteria and Firmicutes and the predominant genera included Actinopolyspora, Gracilibacillus, Halomonas, Nocardiopsis, and Streptomyces. To our knowledge, this is the first time that members of phylum Rhodothermaeota were identified in sediment samples from a salt lake.
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spelling pubmed-73512562020-07-22 Isolation and diversity of sediment bacteria in the hypersaline aiding lake, China Guan, Tong-Wei Lin, Yi-Jin Ou, Meng-Ying Chen, Ke-Bao PLoS One Research Article Halophiles are relatively unexplored as potential sources of novel species. However, little is known about the culturable bacterial diversity thrive in hypersaline lakes. In this work, a total of 343 bacteria from sediment samples of Aiding Lake, China, were isolated using nine different media supplemented with 5% or 15% (w/v) NaCl. The number of species and genera of bacteria recovered from the different media varied, indicating the need to optimize the isolation conditions. The results showed an unexpected level of bacterial diversity, with four phyla (Actinobacteria, Firmicutes, Proteobacteria, and Rhodothermaeota), fourteen orders (Actinopolysporales, Alteromonadales, Bacillales, Balneolales, Chromatiales, Glycomycetales, Jiangellales, Micrococcales, Micromonosporales, Oceanospirillales, Pseudonocardiales, Rhizobiales, Streptomycetales, and Streptosporangiales), including 17 families, 43 genera (including two novel genera), and 71 species (including four novel species). The predominant phyla included Actinobacteria and Firmicutes and the predominant genera included Actinopolyspora, Gracilibacillus, Halomonas, Nocardiopsis, and Streptomyces. To our knowledge, this is the first time that members of phylum Rhodothermaeota were identified in sediment samples from a salt lake. Public Library of Science 2020-07-10 /pmc/articles/PMC7351256/ /pubmed/32649724 http://dx.doi.org/10.1371/journal.pone.0236006 Text en © 2020 Guan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Guan, Tong-Wei
Lin, Yi-Jin
Ou, Meng-Ying
Chen, Ke-Bao
Isolation and diversity of sediment bacteria in the hypersaline aiding lake, China
title Isolation and diversity of sediment bacteria in the hypersaline aiding lake, China
title_full Isolation and diversity of sediment bacteria in the hypersaline aiding lake, China
title_fullStr Isolation and diversity of sediment bacteria in the hypersaline aiding lake, China
title_full_unstemmed Isolation and diversity of sediment bacteria in the hypersaline aiding lake, China
title_short Isolation and diversity of sediment bacteria in the hypersaline aiding lake, China
title_sort isolation and diversity of sediment bacteria in the hypersaline aiding lake, china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351256/
https://www.ncbi.nlm.nih.gov/pubmed/32649724
http://dx.doi.org/10.1371/journal.pone.0236006
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