Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis

Insertions and deletions (InDels) are frequently observed in natural protein evolution, yet their potential remains untapped in laboratory evolution. Here we introduce a transposon-based mutagenesis approach (TRIAD) to generate libraries of random variants with short in-frame InDels, and screen TRIA...

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Detalles Bibliográficos
Autores principales: Emond, Stephane, Petek, Maya, Kay, Emily J., Heames, Brennen, Devenish, Sean R. A., Tokuriki, Nobuhiko, Hollfelder, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351745/
https://www.ncbi.nlm.nih.gov/pubmed/32651386
http://dx.doi.org/10.1038/s41467-020-17061-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351745/
https://www.ncbi.nlm.nih.gov/pubmed/32651386
http://dx.doi.org/10.1038/s41467-020-17061-3

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