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Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis
Insertions and deletions (InDels) are frequently observed in natural protein evolution, yet their potential remains untapped in laboratory evolution. Here we introduce a transposon-based mutagenesis approach (TRIAD) to generate libraries of random variants with short in-frame InDels, and screen TRIA...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351745/ https://www.ncbi.nlm.nih.gov/pubmed/32651386 http://dx.doi.org/10.1038/s41467-020-17061-3 |