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Dietary Supplementation with Dunaliella Tertiolecta Prevents Whitening of Brown Fat and Controls Diet-Induced Obesity at Thermoneutrality in Mice

We investigated the effect of evodiamine-containing microalga Dunaliella tertiolecta (DT) on the prevention of diet-induced obesity in a thermoneutral C57BL/6J male (30 °C). It attenuates the activity of brown adipose tissue (BAT), which accelerates diet-induced obesity. Nine-week-old mice were fed...

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Autores principales: Yamashita, Yukari, Takeuchi, Tamaki, Endo, Yuki, Goto, Ayumi, Sakaki, Setsuko, Yamaguchi, Yuji, Takenaka, Hiroyuki, Yamashita, Hitoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352257/
https://www.ncbi.nlm.nih.gov/pubmed/32516922
http://dx.doi.org/10.3390/nu12061686
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author Yamashita, Yukari
Takeuchi, Tamaki
Endo, Yuki
Goto, Ayumi
Sakaki, Setsuko
Yamaguchi, Yuji
Takenaka, Hiroyuki
Yamashita, Hitoshi
author_facet Yamashita, Yukari
Takeuchi, Tamaki
Endo, Yuki
Goto, Ayumi
Sakaki, Setsuko
Yamaguchi, Yuji
Takenaka, Hiroyuki
Yamashita, Hitoshi
author_sort Yamashita, Yukari
collection PubMed
description We investigated the effect of evodiamine-containing microalga Dunaliella tertiolecta (DT) on the prevention of diet-induced obesity in a thermoneutral C57BL/6J male (30 °C). It attenuates the activity of brown adipose tissue (BAT), which accelerates diet-induced obesity. Nine-week-old mice were fed a high-fat diet supplemented with 10 g (Low group) or 25 g (High group) DT powder per kg food for 12 weeks. Compared to control mice without DT supplementation, body weight gain was significantly reduced in the High group with no difference in food intake. Tissue analyses indicated maintenance of multilocular morphology in BAT and reduced fat deposition in liver in DT-supplemented mice. Molecular analysis showed a significant decrease in mammalian target of rapamycin−ribosomal S6 protein kinase signaling pathway in white adipose tissue and upregulation in mRNA expression of brown fat-associated genes including fibroblast growth factor-21 (Fgf21) and uncoupling protein 1 (Ucp1) in BAT in the High group compared to the control. In the experiments using C3H10T1/2 adipocytes, DT extract upregulated mRNA expression of brown fat-associated genes in dose-dependent and time-dependent manners, accompanied by a significant increase in secreted FGF21 levels. Our data show the ability of DT as a nutraceutical to prevent brown fat attenuation and diet-induced obesity in vivo.
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spelling pubmed-73522572020-07-21 Dietary Supplementation with Dunaliella Tertiolecta Prevents Whitening of Brown Fat and Controls Diet-Induced Obesity at Thermoneutrality in Mice Yamashita, Yukari Takeuchi, Tamaki Endo, Yuki Goto, Ayumi Sakaki, Setsuko Yamaguchi, Yuji Takenaka, Hiroyuki Yamashita, Hitoshi Nutrients Article We investigated the effect of evodiamine-containing microalga Dunaliella tertiolecta (DT) on the prevention of diet-induced obesity in a thermoneutral C57BL/6J male (30 °C). It attenuates the activity of brown adipose tissue (BAT), which accelerates diet-induced obesity. Nine-week-old mice were fed a high-fat diet supplemented with 10 g (Low group) or 25 g (High group) DT powder per kg food for 12 weeks. Compared to control mice without DT supplementation, body weight gain was significantly reduced in the High group with no difference in food intake. Tissue analyses indicated maintenance of multilocular morphology in BAT and reduced fat deposition in liver in DT-supplemented mice. Molecular analysis showed a significant decrease in mammalian target of rapamycin−ribosomal S6 protein kinase signaling pathway in white adipose tissue and upregulation in mRNA expression of brown fat-associated genes including fibroblast growth factor-21 (Fgf21) and uncoupling protein 1 (Ucp1) in BAT in the High group compared to the control. In the experiments using C3H10T1/2 adipocytes, DT extract upregulated mRNA expression of brown fat-associated genes in dose-dependent and time-dependent manners, accompanied by a significant increase in secreted FGF21 levels. Our data show the ability of DT as a nutraceutical to prevent brown fat attenuation and diet-induced obesity in vivo. MDPI 2020-06-05 /pmc/articles/PMC7352257/ /pubmed/32516922 http://dx.doi.org/10.3390/nu12061686 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yamashita, Yukari
Takeuchi, Tamaki
Endo, Yuki
Goto, Ayumi
Sakaki, Setsuko
Yamaguchi, Yuji
Takenaka, Hiroyuki
Yamashita, Hitoshi
Dietary Supplementation with Dunaliella Tertiolecta Prevents Whitening of Brown Fat and Controls Diet-Induced Obesity at Thermoneutrality in Mice
title Dietary Supplementation with Dunaliella Tertiolecta Prevents Whitening of Brown Fat and Controls Diet-Induced Obesity at Thermoneutrality in Mice
title_full Dietary Supplementation with Dunaliella Tertiolecta Prevents Whitening of Brown Fat and Controls Diet-Induced Obesity at Thermoneutrality in Mice
title_fullStr Dietary Supplementation with Dunaliella Tertiolecta Prevents Whitening of Brown Fat and Controls Diet-Induced Obesity at Thermoneutrality in Mice
title_full_unstemmed Dietary Supplementation with Dunaliella Tertiolecta Prevents Whitening of Brown Fat and Controls Diet-Induced Obesity at Thermoneutrality in Mice
title_short Dietary Supplementation with Dunaliella Tertiolecta Prevents Whitening of Brown Fat and Controls Diet-Induced Obesity at Thermoneutrality in Mice
title_sort dietary supplementation with dunaliella tertiolecta prevents whitening of brown fat and controls diet-induced obesity at thermoneutrality in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352257/
https://www.ncbi.nlm.nih.gov/pubmed/32516922
http://dx.doi.org/10.3390/nu12061686
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