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Immunomodulatory and Antioxidant Properties of Wheat Gluten Protein Hydrolysates in Human Peripheral Blood Mononuclear Cells

Peptides from several plant food proteins not only maintain the nutritional values of the original protein and decrease the environmental impact of animal agriculture, but also exert biological activities with significant health-beneficial effects. Wheat is the most important food grain source in th...

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Autores principales: Cruz-Chamorro, Ivan, Álvarez-Sánchez, Nuria, Santos-Sánchez, Guillermo, Pedroche, Justo, Fernández-Pachón, María-Soledad, Millán, Francisco, Millán-Linares, María Carmen, Lardone, Patricia Judith, Bejarano, Ignacio, Guerrero, Juan Miguel, Carrillo-Vico, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352691/
https://www.ncbi.nlm.nih.gov/pubmed/32512720
http://dx.doi.org/10.3390/nu12061673
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author Cruz-Chamorro, Ivan
Álvarez-Sánchez, Nuria
Santos-Sánchez, Guillermo
Pedroche, Justo
Fernández-Pachón, María-Soledad
Millán, Francisco
Millán-Linares, María Carmen
Lardone, Patricia Judith
Bejarano, Ignacio
Guerrero, Juan Miguel
Carrillo-Vico, Antonio
author_facet Cruz-Chamorro, Ivan
Álvarez-Sánchez, Nuria
Santos-Sánchez, Guillermo
Pedroche, Justo
Fernández-Pachón, María-Soledad
Millán, Francisco
Millán-Linares, María Carmen
Lardone, Patricia Judith
Bejarano, Ignacio
Guerrero, Juan Miguel
Carrillo-Vico, Antonio
author_sort Cruz-Chamorro, Ivan
collection PubMed
description Peptides from several plant food proteins not only maintain the nutritional values of the original protein and decrease the environmental impact of animal agriculture, but also exert biological activities with significant health-beneficial effects. Wheat is the most important food grain source in the world. However, negative attention on wheat-based products has arose due to the role of gluten in celiac disease. A controlled enzymatic hydrolysis could reduce the antigenicity of wheat gluten protein hydrolysates (WGPHs). Therefore, the aims of the present study were to evaluate the effects of the in vitro administration of Alcalase-generated WGPHs on the immunological and antioxidant responses of human peripheral blood mononuclear cells (PBMCs) from 39 healthy subjects. WGPH treatment reduced cell proliferation and the production of the Type 1 T helper (Th1) and Th17 pro-inflammatory cytokines IFN-γ and IL-17, respectively. WPGHs also improved the cellular anti-inflammatory microenvironment, increasing Th2/Th1 and Th2/Th17 balances. Additionally, WGPHs improved global antioxidant capacity, increased levels of the reduced form of glutathione and reduced nitric oxide production. These findings, not previously reported, highlight the beneficial capacity of these vegetable protein hydrolysates, which might represent an effective alternative in functional food generation.
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spelling pubmed-73526912020-07-21 Immunomodulatory and Antioxidant Properties of Wheat Gluten Protein Hydrolysates in Human Peripheral Blood Mononuclear Cells Cruz-Chamorro, Ivan Álvarez-Sánchez, Nuria Santos-Sánchez, Guillermo Pedroche, Justo Fernández-Pachón, María-Soledad Millán, Francisco Millán-Linares, María Carmen Lardone, Patricia Judith Bejarano, Ignacio Guerrero, Juan Miguel Carrillo-Vico, Antonio Nutrients Article Peptides from several plant food proteins not only maintain the nutritional values of the original protein and decrease the environmental impact of animal agriculture, but also exert biological activities with significant health-beneficial effects. Wheat is the most important food grain source in the world. However, negative attention on wheat-based products has arose due to the role of gluten in celiac disease. A controlled enzymatic hydrolysis could reduce the antigenicity of wheat gluten protein hydrolysates (WGPHs). Therefore, the aims of the present study were to evaluate the effects of the in vitro administration of Alcalase-generated WGPHs on the immunological and antioxidant responses of human peripheral blood mononuclear cells (PBMCs) from 39 healthy subjects. WGPH treatment reduced cell proliferation and the production of the Type 1 T helper (Th1) and Th17 pro-inflammatory cytokines IFN-γ and IL-17, respectively. WPGHs also improved the cellular anti-inflammatory microenvironment, increasing Th2/Th1 and Th2/Th17 balances. Additionally, WGPHs improved global antioxidant capacity, increased levels of the reduced form of glutathione and reduced nitric oxide production. These findings, not previously reported, highlight the beneficial capacity of these vegetable protein hydrolysates, which might represent an effective alternative in functional food generation. MDPI 2020-06-04 /pmc/articles/PMC7352691/ /pubmed/32512720 http://dx.doi.org/10.3390/nu12061673 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cruz-Chamorro, Ivan
Álvarez-Sánchez, Nuria
Santos-Sánchez, Guillermo
Pedroche, Justo
Fernández-Pachón, María-Soledad
Millán, Francisco
Millán-Linares, María Carmen
Lardone, Patricia Judith
Bejarano, Ignacio
Guerrero, Juan Miguel
Carrillo-Vico, Antonio
Immunomodulatory and Antioxidant Properties of Wheat Gluten Protein Hydrolysates in Human Peripheral Blood Mononuclear Cells
title Immunomodulatory and Antioxidant Properties of Wheat Gluten Protein Hydrolysates in Human Peripheral Blood Mononuclear Cells
title_full Immunomodulatory and Antioxidant Properties of Wheat Gluten Protein Hydrolysates in Human Peripheral Blood Mononuclear Cells
title_fullStr Immunomodulatory and Antioxidant Properties of Wheat Gluten Protein Hydrolysates in Human Peripheral Blood Mononuclear Cells
title_full_unstemmed Immunomodulatory and Antioxidant Properties of Wheat Gluten Protein Hydrolysates in Human Peripheral Blood Mononuclear Cells
title_short Immunomodulatory and Antioxidant Properties of Wheat Gluten Protein Hydrolysates in Human Peripheral Blood Mononuclear Cells
title_sort immunomodulatory and antioxidant properties of wheat gluten protein hydrolysates in human peripheral blood mononuclear cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352691/
https://www.ncbi.nlm.nih.gov/pubmed/32512720
http://dx.doi.org/10.3390/nu12061673
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