Cargando…
Effect of Biofilm Formation by Lactobacillus plantarum on the Malolactic Fermentation in Model Wine
Biofilm life-style of Lactobacillus plantarum (L. plantarum) strains was evaluated in vitro as a new and suitable biotechnological strategy to assure L-malic acid conversion in wine stress conditions. Sixty-eight L. plantarum strains isolated from diverse sources were assessed for their ability to f...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353508/ https://www.ncbi.nlm.nih.gov/pubmed/32560415 http://dx.doi.org/10.3390/foods9060797 |
_version_ | 1783557892430888960 |
---|---|
author | Pannella, Gianfranco Lombardi, Silvia Jane Coppola, Francesca Vergalito, Franca Iorizzo, Massimo Succi, Mariantonietta Tremonte, Patrizio Iannini, Caterina Sorrentino, Elena Coppola, Raffaele |
author_facet | Pannella, Gianfranco Lombardi, Silvia Jane Coppola, Francesca Vergalito, Franca Iorizzo, Massimo Succi, Mariantonietta Tremonte, Patrizio Iannini, Caterina Sorrentino, Elena Coppola, Raffaele |
author_sort | Pannella, Gianfranco |
collection | PubMed |
description | Biofilm life-style of Lactobacillus plantarum (L. plantarum) strains was evaluated in vitro as a new and suitable biotechnological strategy to assure L-malic acid conversion in wine stress conditions. Sixty-eight L. plantarum strains isolated from diverse sources were assessed for their ability to form biofilm in acid (pH 3.5 or 3.2) or in ethanol (12% or 14%) stress conditions. The effect of incubation times (24 and 72 h) on the biofilm formation was evaluated. The study highlighted that, regardless of isolation source and stress conditions, the ability to form biofilm was strain-dependent. Specifically, two clusters, formed by high and low biofilm producer strains, were identified. Among high producer strains, L. plantarum Lpls22 was chosen as the highest producer strain and cultivated in planktonic form or in biofilm using oak supports. Model wines at 12% of ethanol and pH 3.5 or 3.2 were used to assess planktonic and biofilm cells survival and to evaluate the effect of biofilm on L-malic acid conversion. For cells in planktonic form, a strong survival decay was detected. In contrast, cells in biofilm life-style showed high resistance, assuring a prompt and complete L-malic acid conversion. |
format | Online Article Text |
id | pubmed-7353508 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73535082020-07-15 Effect of Biofilm Formation by Lactobacillus plantarum on the Malolactic Fermentation in Model Wine Pannella, Gianfranco Lombardi, Silvia Jane Coppola, Francesca Vergalito, Franca Iorizzo, Massimo Succi, Mariantonietta Tremonte, Patrizio Iannini, Caterina Sorrentino, Elena Coppola, Raffaele Foods Article Biofilm life-style of Lactobacillus plantarum (L. plantarum) strains was evaluated in vitro as a new and suitable biotechnological strategy to assure L-malic acid conversion in wine stress conditions. Sixty-eight L. plantarum strains isolated from diverse sources were assessed for their ability to form biofilm in acid (pH 3.5 or 3.2) or in ethanol (12% or 14%) stress conditions. The effect of incubation times (24 and 72 h) on the biofilm formation was evaluated. The study highlighted that, regardless of isolation source and stress conditions, the ability to form biofilm was strain-dependent. Specifically, two clusters, formed by high and low biofilm producer strains, were identified. Among high producer strains, L. plantarum Lpls22 was chosen as the highest producer strain and cultivated in planktonic form or in biofilm using oak supports. Model wines at 12% of ethanol and pH 3.5 or 3.2 were used to assess planktonic and biofilm cells survival and to evaluate the effect of biofilm on L-malic acid conversion. For cells in planktonic form, a strong survival decay was detected. In contrast, cells in biofilm life-style showed high resistance, assuring a prompt and complete L-malic acid conversion. MDPI 2020-06-17 /pmc/articles/PMC7353508/ /pubmed/32560415 http://dx.doi.org/10.3390/foods9060797 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pannella, Gianfranco Lombardi, Silvia Jane Coppola, Francesca Vergalito, Franca Iorizzo, Massimo Succi, Mariantonietta Tremonte, Patrizio Iannini, Caterina Sorrentino, Elena Coppola, Raffaele Effect of Biofilm Formation by Lactobacillus plantarum on the Malolactic Fermentation in Model Wine |
title | Effect of Biofilm Formation by Lactobacillus plantarum on the Malolactic Fermentation in Model Wine |
title_full | Effect of Biofilm Formation by Lactobacillus plantarum on the Malolactic Fermentation in Model Wine |
title_fullStr | Effect of Biofilm Formation by Lactobacillus plantarum on the Malolactic Fermentation in Model Wine |
title_full_unstemmed | Effect of Biofilm Formation by Lactobacillus plantarum on the Malolactic Fermentation in Model Wine |
title_short | Effect of Biofilm Formation by Lactobacillus plantarum on the Malolactic Fermentation in Model Wine |
title_sort | effect of biofilm formation by lactobacillus plantarum on the malolactic fermentation in model wine |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353508/ https://www.ncbi.nlm.nih.gov/pubmed/32560415 http://dx.doi.org/10.3390/foods9060797 |
work_keys_str_mv | AT pannellagianfranco effectofbiofilmformationbylactobacillusplantarumonthemalolacticfermentationinmodelwine AT lombardisilviajane effectofbiofilmformationbylactobacillusplantarumonthemalolacticfermentationinmodelwine AT coppolafrancesca effectofbiofilmformationbylactobacillusplantarumonthemalolacticfermentationinmodelwine AT vergalitofranca effectofbiofilmformationbylactobacillusplantarumonthemalolacticfermentationinmodelwine AT iorizzomassimo effectofbiofilmformationbylactobacillusplantarumonthemalolacticfermentationinmodelwine AT succimariantonietta effectofbiofilmformationbylactobacillusplantarumonthemalolacticfermentationinmodelwine AT tremontepatrizio effectofbiofilmformationbylactobacillusplantarumonthemalolacticfermentationinmodelwine AT ianninicaterina effectofbiofilmformationbylactobacillusplantarumonthemalolacticfermentationinmodelwine AT sorrentinoelena effectofbiofilmformationbylactobacillusplantarumonthemalolacticfermentationinmodelwine AT coppolaraffaele effectofbiofilmformationbylactobacillusplantarumonthemalolacticfermentationinmodelwine |