Efficiency and cell viability implications using tip type electroporation in zebrafish sperm cells

Sperm-mediated gene transfer (SMGT) has a potential use for zebrafish transgenesis. However, transfection into fish sperm cells still needs to be improved. The objective was to demonstrate the feasibility of tip type electroporation in zebrafish sperm, showing a protocol that provide high transfecti...

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Detalles Bibliográficos
Autores principales: Daneluz, Larissa O., Acosta, Izani B., Nunes, Leandro S., Blodorn, Eduardo B., Domingues, William B., Martins, Amanda W. S., Dellagostin, Eduardo N., Rassier, Gabriela T., Corcini, Carine D., Fróes, Charles N., Komninou, Eliza R., Varela, Antônio S., Campos, Vinicius F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2020
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356131/
https://www.ncbi.nlm.nih.gov/pubmed/32661869
http://dx.doi.org/10.1007/s11033-020-05658-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356131/
https://www.ncbi.nlm.nih.gov/pubmed/32661869
http://dx.doi.org/10.1007/s11033-020-05658-2

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