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Screening Antibody Libraries with Colony Assay Using scFv-Alkaline Phosphatase Fusion Proteins

Screening antibody libraries is an important step in establishing recombinant monoclonal antibodies. The colony assay can identify positive clones without almost any false-positives; however, its antibody library is smaller than those used in other recombinant screening methods such as phage display...

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Detalles Bibliográficos
Autores principales: Hanyu, Yoshiro, Kato, Mieko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356641/
https://www.ncbi.nlm.nih.gov/pubmed/32599779
http://dx.doi.org/10.3390/molecules25122905