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Screening Antibody Libraries with Colony Assay Using scFv-Alkaline Phosphatase Fusion Proteins
Screening antibody libraries is an important step in establishing recombinant monoclonal antibodies. The colony assay can identify positive clones without almost any false-positives; however, its antibody library is smaller than those used in other recombinant screening methods such as phage display...
Autores principales: | Hanyu, Yoshiro, Kato, Mieko |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356641/ https://www.ncbi.nlm.nih.gov/pubmed/32599779 http://dx.doi.org/10.3390/molecules25122905 |
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