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The Effect of Calcium Buffering and Calcium Sensor Type on the Sensitivity of an Array-Based Bitter Receptor Screening Assay
The genetically encoded calcium sensor protein Cameleon YC3.6 has previously been applied for functional G protein–coupled receptor screening using receptor cell arrays. However, different types of sensors are available, with a wide range in [Ca(2+)] sensitivity, Hill coefficients, calcium binding d...
Autores principales: | Roelse, Margriet, Wehrens, Ron, Henquet, Maurice Gl, Witkamp, Renger F, Hall, Robert D, Jongsma, Maarten A |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357244/ https://www.ncbi.nlm.nih.gov/pubmed/31278864 http://dx.doi.org/10.1093/chemse/bjz044 |
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