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Classification of 16S rRNA reads is improved using a niche-specific database constructed by near-full length sequencing

Surveys of microbial populations in environmental niches of interest often utilize sequence variation in the gene encoding the ribosomal small subunit (the 16S rRNA gene). Generally, these surveys target the 16S genes using semi-degenerate primers to amplify portions of a subset of bacterial species...

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Detalles Bibliográficos
Autores principales: Myer, Phillip R., McDaneld, Tara G., Kuehn, Larry A., Dedonder, Keith D., Apley, Michael D., Capik, Sarah F., Lubbers, Brian V., Harhay, Gregory P., Harhay, Dayna M., Keele, John W., Henniger, Madison T., Clemmons, Brooke A., Smith, Timothy P. L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357769/
https://www.ncbi.nlm.nih.gov/pubmed/32658916
http://dx.doi.org/10.1371/journal.pone.0235498