Positive Selection of Specific Antibodies Produced against Fusion Proteins

A method for the positive selection of specific antibodies for target proteins expressed as fusion proteins for the production of antiserum is presented. As proof of concept, the fusion protein FLAG::His::GFP::His::FLAG was expressed in Escherichia coli, purified, and used for the immunization of ra...

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Detalles Bibliográficos
Autores principales: Kramberger-Kaplan, Lukas, Austerlitz, Tina, Bohlmann, Holger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359703/
https://www.ncbi.nlm.nih.gov/pubmed/32397084
http://dx.doi.org/10.3390/mps3020037

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359703/
https://www.ncbi.nlm.nih.gov/pubmed/32397084
http://dx.doi.org/10.3390/mps3020037

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