Analysis of the Level of Plasmid-Derived mRNA in the Presence of Residual Plasmid DNA by Two-Step Quantitative RT-PCR

In transfection experiments with mammalian cells aiming to overexpress a specific protein, it is often necessary to correctly quantify the level of the recombinant and the corresponding endogenous mRNA. In our case, mouse calvarial osteoblasts were transfected with a vector containing the complete P...

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Detalles Bibliográficos
Autores principales: Ahlemeyer, Barbara, Colasante, Claudia, Baumgart-Vogt, Eveline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359704/
https://www.ncbi.nlm.nih.gov/pubmed/32456168
http://dx.doi.org/10.3390/mps3020040

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359704/
https://www.ncbi.nlm.nih.gov/pubmed/32456168
http://dx.doi.org/10.3390/mps3020040

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