Empowering a Methanol-Dependent Escherichia coli via Adaptive Evolution Using a High-Throughput Microbial Microdroplet Culture System

Recently, a methanol-essential Escherichia coli was constructed; this strain is highly dependent on a supply of gluconate as a co-substrate for growth. Adaptive laboratory evolution is commonly applied to obtain mutants with specific phenotypes under certain selected pressure. However, conventional...

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Detalles Bibliográficos
Autores principales: Wang, Jia, Jian, Xingjin, Xing, Xin-Hui, Zhang, Chong, Fei, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7363950/
https://www.ncbi.nlm.nih.gov/pubmed/32733857
http://dx.doi.org/10.3389/fbioe.2020.00570

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7363950/
https://www.ncbi.nlm.nih.gov/pubmed/32733857
http://dx.doi.org/10.3389/fbioe.2020.00570

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