MSI-2小干扰RNA对急性髓系白血病细胞株THP-1细胞增殖及NUMB表达的影响

OBJECTIVE: To investigate the effect of small interfering RNA (siRNA) for MSI-2 on the growth, apoptosis and NUMB expression of THP-1 cells. METHODS: Three siRNA for MSI-2 gene was designed and transfected into THP-1 cells. The cell inhibition, colony formation and apoptosis were determined. The protein expression of NUMB, caspase-3 and PARP were detected by Western blotting. RESULTS: After MSI-2 expression of THP-1 cells was down-regulated for 24 hours, cell inhibition of siRNA MSI-2 group was (47.89±7.64)%, obviously higher than that of negative control group (P=0.005). After 9 days, cell colony count of siRNA MSI-2 group was 7.50±1.53, also lower than that of negative control group (35.75 ± 7.46, P<0.001). In addition, apoptotic rates of siRNA MSI-2 group at 24 hours [(15.22±1.52)%] and 48 hours [(33.83±3.96)%] were significantly higher than those of negative control group (P=0.008 and P=0.001, respectively). Accordingly, activations of caspase-3 and PARP and increased NUMB were observed in siRNA MSI-2 group. CONCLUSION: siRNA for MSI-2 gene could increase the expressions of NUMB to inhibit the proliferation and induce apoptosis of THP-1 cells.