Protective Activities of Dendrobium huoshanense C. Z. Tang et S. J. Cheng Polysaccharide against High-Cholesterol Diet-Induced Atherosclerosis in Zebrafish

Cardiovascular disease is the highest cause of death, and atherosclerosis (AS) is the primary pathogenesis of many cardiovascular diseases. In this study, we aim to investigate the possible pharmaceutical effects of Dendrobium huoshanense C. Z. Tang et S. J. Cheng polysaccharide (DHP) in AS. We fed...

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Autores principales: Fan, Xiangcheng, Han, Jichun, Zhu, Lijun, Chen, Zhipeng, Li, Jiajing, Gu, Yue, Wang, Feng, Wang, Tao, Yue, Yunyun, Shang, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366212/
https://www.ncbi.nlm.nih.gov/pubmed/32724495
http://dx.doi.org/10.1155/2020/8365056
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author Fan, Xiangcheng
Han, Jichun
Zhu, Lijun
Chen, Zhipeng
Li, Jiajing
Gu, Yue
Wang, Feng
Wang, Tao
Yue, Yunyun
Shang, Jing
author_facet Fan, Xiangcheng
Han, Jichun
Zhu, Lijun
Chen, Zhipeng
Li, Jiajing
Gu, Yue
Wang, Feng
Wang, Tao
Yue, Yunyun
Shang, Jing
author_sort Fan, Xiangcheng
collection PubMed
description Cardiovascular disease is the highest cause of death, and atherosclerosis (AS) is the primary pathogenesis of many cardiovascular diseases. In this study, we aim to investigate the possible pharmaceutical effects of Dendrobium huoshanense C. Z. Tang et S. J. Cheng polysaccharide (DHP) in AS. We fed zebrafish with high-cholesterol diet (HCD) to establish a zebrafish AS model and treated with DHP and observed plaque formation and neutrophil counts under a fluorescence microscope. Next, a parallel flow chamber was utilized to establish low shear stress- (LSS-) induced endothelial cell (EC) dysfunction model. We observed that DHP significantly improved HCD-induced lipid deposition, oxidative stress, and inflammatory response, mainly showing that DHP significantly increased superoxide dismutase (SOD) activity, decreased plaque formation, and decreased neutrophil recruitment and the levels of total cholesterol (TC), triglyceride (TG), malondialdehyde (MDA), and reactive oxygen species (ROS). Furthermore, DHP significantly improved LSS-induced oxidative stress and EC dysfunction. Our results indicated that DHP can exert treatment effects on AS, which may attribute to its hypolipidemic, antioxidant, anti-inflammatory activities and improving LSS-induced EC dysfunction. DHP has promising potential for further development as a functional natural medicine source targeted at AS prevention.
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spelling pubmed-73662122020-07-27 Protective Activities of Dendrobium huoshanense C. Z. Tang et S. J. Cheng Polysaccharide against High-Cholesterol Diet-Induced Atherosclerosis in Zebrafish Fan, Xiangcheng Han, Jichun Zhu, Lijun Chen, Zhipeng Li, Jiajing Gu, Yue Wang, Feng Wang, Tao Yue, Yunyun Shang, Jing Oxid Med Cell Longev Research Article Cardiovascular disease is the highest cause of death, and atherosclerosis (AS) is the primary pathogenesis of many cardiovascular diseases. In this study, we aim to investigate the possible pharmaceutical effects of Dendrobium huoshanense C. Z. Tang et S. J. Cheng polysaccharide (DHP) in AS. We fed zebrafish with high-cholesterol diet (HCD) to establish a zebrafish AS model and treated with DHP and observed plaque formation and neutrophil counts under a fluorescence microscope. Next, a parallel flow chamber was utilized to establish low shear stress- (LSS-) induced endothelial cell (EC) dysfunction model. We observed that DHP significantly improved HCD-induced lipid deposition, oxidative stress, and inflammatory response, mainly showing that DHP significantly increased superoxide dismutase (SOD) activity, decreased plaque formation, and decreased neutrophil recruitment and the levels of total cholesterol (TC), triglyceride (TG), malondialdehyde (MDA), and reactive oxygen species (ROS). Furthermore, DHP significantly improved LSS-induced oxidative stress and EC dysfunction. Our results indicated that DHP can exert treatment effects on AS, which may attribute to its hypolipidemic, antioxidant, anti-inflammatory activities and improving LSS-induced EC dysfunction. DHP has promising potential for further development as a functional natural medicine source targeted at AS prevention. Hindawi 2020-07-08 /pmc/articles/PMC7366212/ /pubmed/32724495 http://dx.doi.org/10.1155/2020/8365056 Text en Copyright © 2020 Xiangcheng Fan et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Fan, Xiangcheng
Han, Jichun
Zhu, Lijun
Chen, Zhipeng
Li, Jiajing
Gu, Yue
Wang, Feng
Wang, Tao
Yue, Yunyun
Shang, Jing
Protective Activities of Dendrobium huoshanense C. Z. Tang et S. J. Cheng Polysaccharide against High-Cholesterol Diet-Induced Atherosclerosis in Zebrafish
title Protective Activities of Dendrobium huoshanense C. Z. Tang et S. J. Cheng Polysaccharide against High-Cholesterol Diet-Induced Atherosclerosis in Zebrafish
title_full Protective Activities of Dendrobium huoshanense C. Z. Tang et S. J. Cheng Polysaccharide against High-Cholesterol Diet-Induced Atherosclerosis in Zebrafish
title_fullStr Protective Activities of Dendrobium huoshanense C. Z. Tang et S. J. Cheng Polysaccharide against High-Cholesterol Diet-Induced Atherosclerosis in Zebrafish
title_full_unstemmed Protective Activities of Dendrobium huoshanense C. Z. Tang et S. J. Cheng Polysaccharide against High-Cholesterol Diet-Induced Atherosclerosis in Zebrafish
title_short Protective Activities of Dendrobium huoshanense C. Z. Tang et S. J. Cheng Polysaccharide against High-Cholesterol Diet-Induced Atherosclerosis in Zebrafish
title_sort protective activities of dendrobium huoshanense c. z. tang et s. j. cheng polysaccharide against high-cholesterol diet-induced atherosclerosis in zebrafish
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366212/
https://www.ncbi.nlm.nih.gov/pubmed/32724495
http://dx.doi.org/10.1155/2020/8365056
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