Argonaute binding within 3′-untranslated regions poorly predicts gene repression

Despite two decades of study, the full scope of RNAi in mammalian cells has remained obscure. Here we combine: (i) Knockout of argonaute (AGO) variants; (ii) RNA sequencing analysis of gene expression changes and (iii) Enhanced Crosslinking Immunoprecipitation Sequencing (eCLIP-seq) using anti-AGO2...

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Detalles Bibliográficos
Autores principales: Chu, Yongjun, Kilikevicius, Audrius, Liu, Jing, Johnson, Krystal C, Yokota, Shinnichi, Corey, David R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
RNA and RNA-protein complexes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367155/
https://www.ncbi.nlm.nih.gov/pubmed/32501500
http://dx.doi.org/10.1093/nar/gkaa478

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367155/
https://www.ncbi.nlm.nih.gov/pubmed/32501500
http://dx.doi.org/10.1093/nar/gkaa478

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