CLUE: a bioinformatic and wet-lab pipeline for multiplexed cloning of custom sgRNA libraries

The systematic perturbation of genomes using CRISPR/Cas9 deciphers gene function at an unprecedented rate, depth and ease. Commercially available sgRNA libraries typically contain tens of thousands of pre-defined constructs, resulting in a complexity challenging to handle. In contrast, custom sgRNA...

Descripción completa

Detalles Bibliográficos
Autores principales: Becker, Martin, Noll-Puchta, Heidi, Amend, Diana, Nolte, Florian, Fuchs, Christiane, Jeremias, Irmela, Braun, Christian J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367185/
https://www.ncbi.nlm.nih.gov/pubmed/32479629
http://dx.doi.org/10.1093/nar/gkaa459

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367185/
https://www.ncbi.nlm.nih.gov/pubmed/32479629
http://dx.doi.org/10.1093/nar/gkaa459

Ejemplares similares