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CLUE: a bioinformatic and wet-lab pipeline for multiplexed cloning of custom sgRNA libraries
The systematic perturbation of genomes using CRISPR/Cas9 deciphers gene function at an unprecedented rate, depth and ease. Commercially available sgRNA libraries typically contain tens of thousands of pre-defined constructs, resulting in a complexity challenging to handle. In contrast, custom sgRNA...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367185/ https://www.ncbi.nlm.nih.gov/pubmed/32479629 http://dx.doi.org/10.1093/nar/gkaa459 |