Engineered CRISPR/Cas9 enzymes improve discrimination by slowing DNA cleavage to allow release of off-target DNA

CRISPR/Cas9 is a programmable genome editing tool widely used for biological applications and engineered Cas9s have increased discrimination against off-target cleavage compared with wild-type Streptococcus pyogenes (SpCas9) in vivo. To understand the basis for improved discrimination against off-ta...

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Detalles Bibliográficos
Autores principales: Liu, Mu-Sen, Gong, Shanzhong, Yu, Helen-Hong, Jung, Kyungseok, Johnson, Kenneth A., Taylor, David W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367838/
https://www.ncbi.nlm.nih.gov/pubmed/32681021
http://dx.doi.org/10.1038/s41467-020-17411-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367838/
https://www.ncbi.nlm.nih.gov/pubmed/32681021
http://dx.doi.org/10.1038/s41467-020-17411-1

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