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Engineered CRISPR/Cas9 enzymes improve discrimination by slowing DNA cleavage to allow release of off-target DNA

CRISPR/Cas9 is a programmable genome editing tool widely used for biological applications and engineered Cas9s have increased discrimination against off-target cleavage compared with wild-type Streptococcus pyogenes (SpCas9) in vivo. To understand the basis for improved discrimination against off-ta...

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Detalles Bibliográficos
Autores principales:	Liu, Mu-Sen, Gong, Shanzhong, Yu, Helen-Hong, Jung, Kyungseok, Johnson, Kenneth A., Taylor, David W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367838/ https://www.ncbi.nlm.nih.gov/pubmed/32681021 http://dx.doi.org/10.1038/s41467-020-17411-1

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