Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment

CRISPR effectors, which comprise a CRISPR-Cas protein and a guide (g)RNA derived from the bacterial immune system, are widely used for target-specific genome editing. When the gRNA recognizes genomic loci with sequences that are similar to the target, deleterious mutations can occur. Off-target muta...

Descripción completa

Detalles Bibliográficos
Autores principales: Kang, Seung-Hun, Lee, Wi-jae, An, Ju-Hyun, Lee, Jong-Hee, Kim, Young-Hyun, Kim, Hanseop, Oh, Yeounsun, Park, Young-Ho, Jin, Yeung Bae, Jun, Bong-Hyun, Hur, Junho K., Kim, Sun-Uk, Lee, Seung Hwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368065/
https://www.ncbi.nlm.nih.gov/pubmed/32681048
http://dx.doi.org/10.1038/s41467-020-17418-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368065/
https://www.ncbi.nlm.nih.gov/pubmed/32681048
http://dx.doi.org/10.1038/s41467-020-17418-8

Ejemplares similares