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Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment
CRISPR effectors, which comprise a CRISPR-Cas protein and a guide (g)RNA derived from the bacterial immune system, are widely used for target-specific genome editing. When the gRNA recognizes genomic loci with sequences that are similar to the target, deleterious mutations can occur. Off-target muta...
Autores principales: | Kang, Seung-Hun, Lee, Wi-jae, An, Ju-Hyun, Lee, Jong-Hee, Kim, Young-Hyun, Kim, Hanseop, Oh, Yeounsun, Park, Young-Ho, Jin, Yeung Bae, Jun, Bong-Hyun, Hur, Junho K., Kim, Sun-Uk, Lee, Seung Hwan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368065/ https://www.ncbi.nlm.nih.gov/pubmed/32681048 http://dx.doi.org/10.1038/s41467-020-17418-8 |
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