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Single-Cell Sequencing of Peripheral Mononuclear Cells Reveals Distinct Immune Response Landscapes of COVID-19 and Influenza Patients

The coronavirus disease 2019 (COVID-19) pandemic poses a current world-wide public health threat. However, little is known about its hallmarks compared to other infectious diseases. Here, we report the single-cell transcriptional landscape of longitudinally collected peripheral blood mononuclear cel...

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Autores principales: Zhu, Linnan, Yang, Penghui, Zhao, Yingze, Zhuang, Zhenkun, Wang, Zhifeng, Song, Rui, Zhang, Jie, Liu, Chuanyu, Gao, Qianqian, Xu, Qumiao, Wei, Xiaoyu, Sun, Hai-Xi, Ye, Beiwei, Wu, Yanan, Zhang, Ning, Lei, Guanglin, Yu, Lingxiang, Yan, Jin, Diao, Guanghao, Meng, Fanping, Bai, Changqing, Mao, Panyong, Yu, Yeya, Wang, Mingyue, Yuan, Yue, Deng, Qiuting, Li, Ziyi, Huang, Yunting, Hu, Guohai, Liu, Yang, Wang, Xiaoqian, Xu, Ziqian, Liu, Peipei, Bi, Yuhai, Shi, Yi, Zhang, Shaogeng, Chen, Zhihai, Wang, Jian, Xu, Xun, Wu, Guizhen, Wang, Fu-Sheng, Gao, George F., Liu, Longqi, Liu, William J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368915/
https://www.ncbi.nlm.nih.gov/pubmed/32783921
http://dx.doi.org/10.1016/j.immuni.2020.07.009
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author Zhu, Linnan
Yang, Penghui
Zhao, Yingze
Zhuang, Zhenkun
Wang, Zhifeng
Song, Rui
Zhang, Jie
Liu, Chuanyu
Gao, Qianqian
Xu, Qumiao
Wei, Xiaoyu
Sun, Hai-Xi
Ye, Beiwei
Wu, Yanan
Zhang, Ning
Lei, Guanglin
Yu, Lingxiang
Yan, Jin
Diao, Guanghao
Meng, Fanping
Bai, Changqing
Mao, Panyong
Yu, Yeya
Wang, Mingyue
Yuan, Yue
Deng, Qiuting
Li, Ziyi
Huang, Yunting
Hu, Guohai
Liu, Yang
Wang, Xiaoqian
Xu, Ziqian
Liu, Peipei
Bi, Yuhai
Shi, Yi
Zhang, Shaogeng
Chen, Zhihai
Wang, Jian
Xu, Xun
Wu, Guizhen
Wang, Fu-Sheng
Gao, George F.
Liu, Longqi
Liu, William J.
author_facet Zhu, Linnan
Yang, Penghui
Zhao, Yingze
Zhuang, Zhenkun
Wang, Zhifeng
Song, Rui
Zhang, Jie
Liu, Chuanyu
Gao, Qianqian
Xu, Qumiao
Wei, Xiaoyu
Sun, Hai-Xi
Ye, Beiwei
Wu, Yanan
Zhang, Ning
Lei, Guanglin
Yu, Lingxiang
Yan, Jin
Diao, Guanghao
Meng, Fanping
Bai, Changqing
Mao, Panyong
Yu, Yeya
Wang, Mingyue
Yuan, Yue
Deng, Qiuting
Li, Ziyi
Huang, Yunting
Hu, Guohai
Liu, Yang
Wang, Xiaoqian
Xu, Ziqian
Liu, Peipei
Bi, Yuhai
Shi, Yi
Zhang, Shaogeng
Chen, Zhihai
Wang, Jian
Xu, Xun
Wu, Guizhen
Wang, Fu-Sheng
Gao, George F.
Liu, Longqi
Liu, William J.
author_sort Zhu, Linnan
collection PubMed
description The coronavirus disease 2019 (COVID-19) pandemic poses a current world-wide public health threat. However, little is known about its hallmarks compared to other infectious diseases. Here, we report the single-cell transcriptional landscape of longitudinally collected peripheral blood mononuclear cells (PBMCs) in both COVID-19- and influenza A virus (IAV)-infected patients. We observed increase of plasma cells in both COVID-19 and IAV patients and XIAP associated factor 1 (XAF1)-, tumor necrosis factor (TNF)-, and FAS-induced T cell apoptosis in COVID-19 patients. Further analyses revealed distinct signaling pathways activated in COVID-19 (STAT1 and IRF3) versus IAV (STAT3 and NFκB) patients and substantial differences in the expression of key factors. These factors include relatively increase of interleukin (IL)6R and IL6ST expression in COVID-19 patients but similarly increased IL-6 concentrations compared to IAV patients, supporting the clinical observations of increased proinflammatory cytokines in COVID-19 patients. Thus, we provide the landscape of PBMCs and unveil distinct immune response pathways in COVID-19 and IAV patients.
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spelling pubmed-73689152020-07-20 Single-Cell Sequencing of Peripheral Mononuclear Cells Reveals Distinct Immune Response Landscapes of COVID-19 and Influenza Patients Zhu, Linnan Yang, Penghui Zhao, Yingze Zhuang, Zhenkun Wang, Zhifeng Song, Rui Zhang, Jie Liu, Chuanyu Gao, Qianqian Xu, Qumiao Wei, Xiaoyu Sun, Hai-Xi Ye, Beiwei Wu, Yanan Zhang, Ning Lei, Guanglin Yu, Lingxiang Yan, Jin Diao, Guanghao Meng, Fanping Bai, Changqing Mao, Panyong Yu, Yeya Wang, Mingyue Yuan, Yue Deng, Qiuting Li, Ziyi Huang, Yunting Hu, Guohai Liu, Yang Wang, Xiaoqian Xu, Ziqian Liu, Peipei Bi, Yuhai Shi, Yi Zhang, Shaogeng Chen, Zhihai Wang, Jian Xu, Xun Wu, Guizhen Wang, Fu-Sheng Gao, George F. Liu, Longqi Liu, William J. Immunity Article The coronavirus disease 2019 (COVID-19) pandemic poses a current world-wide public health threat. However, little is known about its hallmarks compared to other infectious diseases. Here, we report the single-cell transcriptional landscape of longitudinally collected peripheral blood mononuclear cells (PBMCs) in both COVID-19- and influenza A virus (IAV)-infected patients. We observed increase of plasma cells in both COVID-19 and IAV patients and XIAP associated factor 1 (XAF1)-, tumor necrosis factor (TNF)-, and FAS-induced T cell apoptosis in COVID-19 patients. Further analyses revealed distinct signaling pathways activated in COVID-19 (STAT1 and IRF3) versus IAV (STAT3 and NFκB) patients and substantial differences in the expression of key factors. These factors include relatively increase of interleukin (IL)6R and IL6ST expression in COVID-19 patients but similarly increased IL-6 concentrations compared to IAV patients, supporting the clinical observations of increased proinflammatory cytokines in COVID-19 patients. Thus, we provide the landscape of PBMCs and unveil distinct immune response pathways in COVID-19 and IAV patients. Elsevier Inc. 2020-09-15 2020-07-19 /pmc/articles/PMC7368915/ /pubmed/32783921 http://dx.doi.org/10.1016/j.immuni.2020.07.009 Text en © 2020 Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Zhu, Linnan
Yang, Penghui
Zhao, Yingze
Zhuang, Zhenkun
Wang, Zhifeng
Song, Rui
Zhang, Jie
Liu, Chuanyu
Gao, Qianqian
Xu, Qumiao
Wei, Xiaoyu
Sun, Hai-Xi
Ye, Beiwei
Wu, Yanan
Zhang, Ning
Lei, Guanglin
Yu, Lingxiang
Yan, Jin
Diao, Guanghao
Meng, Fanping
Bai, Changqing
Mao, Panyong
Yu, Yeya
Wang, Mingyue
Yuan, Yue
Deng, Qiuting
Li, Ziyi
Huang, Yunting
Hu, Guohai
Liu, Yang
Wang, Xiaoqian
Xu, Ziqian
Liu, Peipei
Bi, Yuhai
Shi, Yi
Zhang, Shaogeng
Chen, Zhihai
Wang, Jian
Xu, Xun
Wu, Guizhen
Wang, Fu-Sheng
Gao, George F.
Liu, Longqi
Liu, William J.
Single-Cell Sequencing of Peripheral Mononuclear Cells Reveals Distinct Immune Response Landscapes of COVID-19 and Influenza Patients
title Single-Cell Sequencing of Peripheral Mononuclear Cells Reveals Distinct Immune Response Landscapes of COVID-19 and Influenza Patients
title_full Single-Cell Sequencing of Peripheral Mononuclear Cells Reveals Distinct Immune Response Landscapes of COVID-19 and Influenza Patients
title_fullStr Single-Cell Sequencing of Peripheral Mononuclear Cells Reveals Distinct Immune Response Landscapes of COVID-19 and Influenza Patients
title_full_unstemmed Single-Cell Sequencing of Peripheral Mononuclear Cells Reveals Distinct Immune Response Landscapes of COVID-19 and Influenza Patients
title_short Single-Cell Sequencing of Peripheral Mononuclear Cells Reveals Distinct Immune Response Landscapes of COVID-19 and Influenza Patients
title_sort single-cell sequencing of peripheral mononuclear cells reveals distinct immune response landscapes of covid-19 and influenza patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368915/
https://www.ncbi.nlm.nih.gov/pubmed/32783921
http://dx.doi.org/10.1016/j.immuni.2020.07.009
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