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In Situ Detection of Endogenous HIV Activation by Dynamic Nuclear Polarization NMR and Flow Cytometry

We demonstrate for the first time in-cell dynamic nuclear polarization (DNP) in conjunction with flow cytometry sorting to address the cellular heterogeneity of in-cell samples. Utilizing a green fluorescent protein (GFP) reporter of HIV reactivation, we correlate increased (15)N resonance intensity...

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Detalles Bibliográficos
Autores principales: Overall, Sarah A., Price, Lauren E., Albert, Brice J., Gao, Chukun, Alaniva, Nicholas, Judge, Patrick T., Sesti, Erika L., Wender, Paul A., Kyei, George B., Barnes, Alexander B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7369949/
https://www.ncbi.nlm.nih.gov/pubmed/32629894
http://dx.doi.org/10.3390/ijms21134649