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The Improvement of Bioethanol Production by Pentose-Fermenting Yeasts Isolated from Herbal Preparations, the Gut of Dung Beetles, and Marula Wine

Efficient conversion of pentose sugars to ethanol is important for an economically viable lignocellulosic bioethanol process. Ten yeasts fermenting both D-xylose and L-arabinose were subjected to an adaptation process with L-arabinose as carbon source in a medium containing acetic acid. Four Meyeroz...

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Autores principales: Moremi, Mahlatse Ellias, Jansen Van Rensburg, Elbert Lukas, La Grange, Daniël Coenrad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7374204/
https://www.ncbi.nlm.nih.gov/pubmed/32765609
http://dx.doi.org/10.1155/2020/5670936
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author Moremi, Mahlatse Ellias
Jansen Van Rensburg, Elbert Lukas
La Grange, Daniël Coenrad
author_facet Moremi, Mahlatse Ellias
Jansen Van Rensburg, Elbert Lukas
La Grange, Daniël Coenrad
author_sort Moremi, Mahlatse Ellias
collection PubMed
description Efficient conversion of pentose sugars to ethanol is important for an economically viable lignocellulosic bioethanol process. Ten yeasts fermenting both D-xylose and L-arabinose were subjected to an adaptation process with L-arabinose as carbon source in a medium containing acetic acid. Four Meyerozyma caribbica-adapted strains were able to ferment L-arabinose to ethanol in the presence of 3 g/L acetic acid at 35°C. Meyerozyma caribbica Mu 2.2f fermented L-arabinose to produce 3.0 g/L ethanol compared to the parental strain with 1.0 g/L ethanol in the absence of acetic acid. The adapted M. caribbica Mu 2.2f strain produced 3.6 and 0.8 g/L ethanol on L-arabinose and D-xylose, respectively, in the presence of acetic acid while the parental strain failed to grow. In a bioreactor, the adapted M. caribbica Mu 2.2f strain produced 5.7 g/L ethanol in the presence of 3 g/L acetic acid with an ethanol yield and productivity of 0.338 g/g and 0.158 g/L/h, respectively, at a K(L)a value of 3.3 h(−1). The adapted strain produced 26.7 g/L L-arabitol with a yield of 0.900 g/g at a K(L)a value of 4.9 h(−1).
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spelling pubmed-73742042020-08-05 The Improvement of Bioethanol Production by Pentose-Fermenting Yeasts Isolated from Herbal Preparations, the Gut of Dung Beetles, and Marula Wine Moremi, Mahlatse Ellias Jansen Van Rensburg, Elbert Lukas La Grange, Daniël Coenrad Int J Microbiol Research Article Efficient conversion of pentose sugars to ethanol is important for an economically viable lignocellulosic bioethanol process. Ten yeasts fermenting both D-xylose and L-arabinose were subjected to an adaptation process with L-arabinose as carbon source in a medium containing acetic acid. Four Meyerozyma caribbica-adapted strains were able to ferment L-arabinose to ethanol in the presence of 3 g/L acetic acid at 35°C. Meyerozyma caribbica Mu 2.2f fermented L-arabinose to produce 3.0 g/L ethanol compared to the parental strain with 1.0 g/L ethanol in the absence of acetic acid. The adapted M. caribbica Mu 2.2f strain produced 3.6 and 0.8 g/L ethanol on L-arabinose and D-xylose, respectively, in the presence of acetic acid while the parental strain failed to grow. In a bioreactor, the adapted M. caribbica Mu 2.2f strain produced 5.7 g/L ethanol in the presence of 3 g/L acetic acid with an ethanol yield and productivity of 0.338 g/g and 0.158 g/L/h, respectively, at a K(L)a value of 3.3 h(−1). The adapted strain produced 26.7 g/L L-arabitol with a yield of 0.900 g/g at a K(L)a value of 4.9 h(−1). Hindawi 2020-07-13 /pmc/articles/PMC7374204/ /pubmed/32765609 http://dx.doi.org/10.1155/2020/5670936 Text en Copyright © 2020 Mahlatse Ellias Moremi et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Moremi, Mahlatse Ellias
Jansen Van Rensburg, Elbert Lukas
La Grange, Daniël Coenrad
The Improvement of Bioethanol Production by Pentose-Fermenting Yeasts Isolated from Herbal Preparations, the Gut of Dung Beetles, and Marula Wine
title The Improvement of Bioethanol Production by Pentose-Fermenting Yeasts Isolated from Herbal Preparations, the Gut of Dung Beetles, and Marula Wine
title_full The Improvement of Bioethanol Production by Pentose-Fermenting Yeasts Isolated from Herbal Preparations, the Gut of Dung Beetles, and Marula Wine
title_fullStr The Improvement of Bioethanol Production by Pentose-Fermenting Yeasts Isolated from Herbal Preparations, the Gut of Dung Beetles, and Marula Wine
title_full_unstemmed The Improvement of Bioethanol Production by Pentose-Fermenting Yeasts Isolated from Herbal Preparations, the Gut of Dung Beetles, and Marula Wine
title_short The Improvement of Bioethanol Production by Pentose-Fermenting Yeasts Isolated from Herbal Preparations, the Gut of Dung Beetles, and Marula Wine
title_sort improvement of bioethanol production by pentose-fermenting yeasts isolated from herbal preparations, the gut of dung beetles, and marula wine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7374204/
https://www.ncbi.nlm.nih.gov/pubmed/32765609
http://dx.doi.org/10.1155/2020/5670936
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