Dissecting efficiency of a 5’ rapid amplification of cDNA ends (5’-RACE) approach for profiling T-cell receptor beta repertoire

Deep sequencing of T-cell receptor (TCR) genes is powerful at profiling immune repertoire. To prepare a TCR sequencing library, multiplex polymerase chain reaction (mPCR) is widely applied and is highly efficient. That is, most mPCR products contain the region critical for antigen recognition, which...

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Detalles Bibliográficos
Autores principales: Lin, Yu-Hung, Hung, Sheng-Jou, Chen, Yi-Lin, Lin, Cheng-Han, Kung, Te-Fang, Yeh, Yi-Chun, Tseng, Joseph T., Liu, Tsunglin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377388/
https://www.ncbi.nlm.nih.gov/pubmed/32702062
http://dx.doi.org/10.1371/journal.pone.0236366

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377388/
https://www.ncbi.nlm.nih.gov/pubmed/32702062
http://dx.doi.org/10.1371/journal.pone.0236366

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