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Bleaching correction for DNA measurements in highly diluted solutions using confocal microscopy

Determining the concentration of nucleic acids in biological samples precisely and reliably still is a challenge. In particular when only very small sample quantities are available for analysis, the established fluorescence-based methods give insufficient results. Photobleaching is seen as the main...

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Detalles Bibliográficos
Autores principales: Sparrenberg, Lorenz Tim, Greiner, Benjamin, Mathis, Harald Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377397/
https://www.ncbi.nlm.nih.gov/pubmed/32702012
http://dx.doi.org/10.1371/journal.pone.0231918