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An efficient ORF selection system for DNA fragment libraries based on split beta-lactamase complementation
PCR-based amplification of annotated genes has allowed construction of expression clones at genome-scale using classical and recombination-based cloning technologies. However, genome-scale expression and purification of proteins for down-stream applications is often limited by challenges such as poo...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377443/ https://www.ncbi.nlm.nih.gov/pubmed/32701967 http://dx.doi.org/10.1371/journal.pone.0235853 |