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Altered expression of a quality control protease in E. coli reshapes the in vivo mutational landscape of a model enzyme

Protein mutational landscapes are shaped by the cellular environment, but key factors and their quantitative effects are often unknown. Here we show that Lon, a quality control protease naturally absent in common E. coli expression strains, drastically reshapes the mutational landscape of the metabo...

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Detalles Bibliográficos
Autores principales: Thompson, Samuel, Zhang, Yang, Ingle, Christine, Reynolds, Kimberly A, Kortemme, Tanja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7377907/
https://www.ncbi.nlm.nih.gov/pubmed/32701056
http://dx.doi.org/10.7554/eLife.53476