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New mitochondrial primers for metabarcoding of insects, designed and evaluated using in silico methods
Insect metabarcoding has been mainly based on PCR amplification of short fragments within the “barcoding region” of the gene cytochrome oxidase I (COI). However, because of the variability of this gene, it has been difficult to design good universal PCR primers. Most primers used today are associate...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7379581/ https://www.ncbi.nlm.nih.gov/pubmed/30226026 http://dx.doi.org/10.1111/1755-0998.12942 |