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Neutrophil‐like HL‐60 cells expressing only GFP‐tagged β‐actin exhibit nearly normal motility

Observations of actin dynamics in living cells using fluorescence microscopy have been foundational in the exploration of the mechanisms underlying cell migration. We used CRISPR/Cas9 gene editing to generate neutrophil‐like HL‐60 cell lines expressing GFP‐β‐actin from the endogenous locus (ACTB). I...

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Detalles Bibliográficos
Autores principales: Garner, Rikki M., Skariah, Gemini, Hadjitheodorou, Amalia, Belliveau, Nathan M., Savinov, Andrew, Footer, Matthew J., Theriot, Julie A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383899/
https://www.ncbi.nlm.nih.gov/pubmed/32072765
http://dx.doi.org/10.1002/cm.21603