Nonspecific cleavages arising from reconstitution of trypsin under mildly acidic conditions

Tryptic digestion of proteins followed by liquid chromatography with tandem mass spectrometry analysis is an extensively used approach in proteomics research and biopharmaceutical product characterization, owing to the high level of cleavage fidelity produced with this technique. However, nonspecifi...

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Detalles Bibliográficos
Autores principales: Niu, Ben, Martinelli II, Michael, Jiao, Yang, Wang, Chunlei, Cao, Mingyan, Wang, Jihong, Meinke, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7386593/
https://www.ncbi.nlm.nih.gov/pubmed/32722706
http://dx.doi.org/10.1371/journal.pone.0236740

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7386593/
https://www.ncbi.nlm.nih.gov/pubmed/32722706
http://dx.doi.org/10.1371/journal.pone.0236740

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