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A Polyethylene Glycol-Based Method for Enrichment of Extracellular Vesicles from Culture Supernatant of Human Ovarian Cancer Cell Line A2780 and Body Fluids of High-Grade Serous Carcinoma Patients

OBJECTIVE: This study tried to evaluate whether 8% polyethylene glycol (PEG) 6000 precipitation combined with differential ultracentrifugation (PPDU) was an efficient and practical method for the enrichment and purification of extracellular vesicles (EVs) derived from the culture supernatant of huma...

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Detalles Bibliográficos
Autores principales: Jiao, Ruili, Sun, Shipeng, Gao, Xiaoqiong, Cui, Ran, Cao, Guangming, Wei, Huali, Wang, Shuzhen, Zhang, Zhenyu, Bai, Huimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7386806/
https://www.ncbi.nlm.nih.gov/pubmed/32801874
http://dx.doi.org/10.2147/CMAR.S228288