Characterization of kynurenine pathway in patients with diarrhea-predominant irritable bowel syndrome

Our objectives are to demonstrate whether the kynurenine pathway is activated in diarrhea-type irritable bowel syndrome (IBS-D) patients, and whether the neurotoxic metabolite quinolinic acid (QUIN) is out of balance with the neuroprotective metabolite kynurenic acid (KYNA), and further explore whet...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Pingping, Zheng, Jimin, Bai, Yun, Wang, Dingxin, Cui, Zijin, Li, Yueqin, Zhang, Jian, Wang, Yuzhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388639/
https://www.ncbi.nlm.nih.gov/pubmed/32705857
http://dx.doi.org/10.4081/ejh.2020.3132
_version_ 1783564348147367936
author Li, Pingping
Zheng, Jimin
Bai, Yun
Wang, Dingxin
Cui, Zijin
Li, Yueqin
Zhang, Jian
Wang, Yuzhen
author_facet Li, Pingping
Zheng, Jimin
Bai, Yun
Wang, Dingxin
Cui, Zijin
Li, Yueqin
Zhang, Jian
Wang, Yuzhen
author_sort Li, Pingping
collection PubMed
description Our objectives are to demonstrate whether the kynurenine pathway is activated in diarrhea-type irritable bowel syndrome (IBS-D) patients, and whether the neurotoxic metabolite quinolinic acid (QUIN) is out of balance with the neuroprotective metabolite kynurenic acid (KYNA), and further explore whether this can lead to increased expression of N-methyl D-aspartate receptor 2B (NMDAR2B) in the enteric nervous system and in turn leads to intestinal symptoms and mood disorders. All enrolled healthy controls and patients accepted IBS Symptom Severity Scale (IBS-SSS) score, Self-rating Depression Scale (SDS) and Self-rating Anxiety Scale (SAS) anxiety and depression scores, and also underwent colonoscopy to collect ileum and colonic mucosa specimens. The expression of NMDAR2B in intestinal mucosa was detected by immunofluorescence, and fasting serum was collected to detect the tryptophan, kynurenine (KYN), KYNA and QUIN by high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Our results showed that the KYN pathway of IBS-D patients was activated. The production of QUIN and KYNA was imbalanced and resulting in an increased NMDAR2B for patients with IBS-D, which may be involved in intestinal symptoms and mood disorders of IBS-D.
format Online
Article
Text
id pubmed-7388639
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher PAGEPress Publications, Pavia, Italy
record_format MEDLINE/PubMed
spelling pubmed-73886392020-08-12 Characterization of kynurenine pathway in patients with diarrhea-predominant irritable bowel syndrome Li, Pingping Zheng, Jimin Bai, Yun Wang, Dingxin Cui, Zijin Li, Yueqin Zhang, Jian Wang, Yuzhen Eur J Histochem Article Our objectives are to demonstrate whether the kynurenine pathway is activated in diarrhea-type irritable bowel syndrome (IBS-D) patients, and whether the neurotoxic metabolite quinolinic acid (QUIN) is out of balance with the neuroprotective metabolite kynurenic acid (KYNA), and further explore whether this can lead to increased expression of N-methyl D-aspartate receptor 2B (NMDAR2B) in the enteric nervous system and in turn leads to intestinal symptoms and mood disorders. All enrolled healthy controls and patients accepted IBS Symptom Severity Scale (IBS-SSS) score, Self-rating Depression Scale (SDS) and Self-rating Anxiety Scale (SAS) anxiety and depression scores, and also underwent colonoscopy to collect ileum and colonic mucosa specimens. The expression of NMDAR2B in intestinal mucosa was detected by immunofluorescence, and fasting serum was collected to detect the tryptophan, kynurenine (KYN), KYNA and QUIN by high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Our results showed that the KYN pathway of IBS-D patients was activated. The production of QUIN and KYNA was imbalanced and resulting in an increased NMDAR2B for patients with IBS-D, which may be involved in intestinal symptoms and mood disorders of IBS-D. PAGEPress Publications, Pavia, Italy 2020-06-19 /pmc/articles/PMC7388639/ /pubmed/32705857 http://dx.doi.org/10.4081/ejh.2020.3132 Text en ©Copyright: the Author(s) http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Li, Pingping
Zheng, Jimin
Bai, Yun
Wang, Dingxin
Cui, Zijin
Li, Yueqin
Zhang, Jian
Wang, Yuzhen
Characterization of kynurenine pathway in patients with diarrhea-predominant irritable bowel syndrome
title Characterization of kynurenine pathway in patients with diarrhea-predominant irritable bowel syndrome
title_full Characterization of kynurenine pathway in patients with diarrhea-predominant irritable bowel syndrome
title_fullStr Characterization of kynurenine pathway in patients with diarrhea-predominant irritable bowel syndrome
title_full_unstemmed Characterization of kynurenine pathway in patients with diarrhea-predominant irritable bowel syndrome
title_short Characterization of kynurenine pathway in patients with diarrhea-predominant irritable bowel syndrome
title_sort characterization of kynurenine pathway in patients with diarrhea-predominant irritable bowel syndrome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388639/
https://www.ncbi.nlm.nih.gov/pubmed/32705857
http://dx.doi.org/10.4081/ejh.2020.3132
work_keys_str_mv AT lipingping characterizationofkynureninepathwayinpatientswithdiarrheapredominantirritablebowelsyndrome
AT zhengjimin characterizationofkynureninepathwayinpatientswithdiarrheapredominantirritablebowelsyndrome
AT baiyun characterizationofkynureninepathwayinpatientswithdiarrheapredominantirritablebowelsyndrome
AT wangdingxin characterizationofkynureninepathwayinpatientswithdiarrheapredominantirritablebowelsyndrome
AT cuizijin characterizationofkynureninepathwayinpatientswithdiarrheapredominantirritablebowelsyndrome
AT liyueqin characterizationofkynureninepathwayinpatientswithdiarrheapredominantirritablebowelsyndrome
AT zhangjian characterizationofkynureninepathwayinpatientswithdiarrheapredominantirritablebowelsyndrome
AT wangyuzhen characterizationofkynureninepathwayinpatientswithdiarrheapredominantirritablebowelsyndrome