Characterization of kynurenine pathway in patients with diarrhea-predominant irritable bowel syndrome
Our objectives are to demonstrate whether the kynurenine pathway is activated in diarrhea-type irritable bowel syndrome (IBS-D) patients, and whether the neurotoxic metabolite quinolinic acid (QUIN) is out of balance with the neuroprotective metabolite kynurenic acid (KYNA), and further explore whet...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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PAGEPress Publications, Pavia, Italy
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388639/ https://www.ncbi.nlm.nih.gov/pubmed/32705857 http://dx.doi.org/10.4081/ejh.2020.3132 |
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author | Li, Pingping Zheng, Jimin Bai, Yun Wang, Dingxin Cui, Zijin Li, Yueqin Zhang, Jian Wang, Yuzhen |
author_facet | Li, Pingping Zheng, Jimin Bai, Yun Wang, Dingxin Cui, Zijin Li, Yueqin Zhang, Jian Wang, Yuzhen |
author_sort | Li, Pingping |
collection | PubMed |
description | Our objectives are to demonstrate whether the kynurenine pathway is activated in diarrhea-type irritable bowel syndrome (IBS-D) patients, and whether the neurotoxic metabolite quinolinic acid (QUIN) is out of balance with the neuroprotective metabolite kynurenic acid (KYNA), and further explore whether this can lead to increased expression of N-methyl D-aspartate receptor 2B (NMDAR2B) in the enteric nervous system and in turn leads to intestinal symptoms and mood disorders. All enrolled healthy controls and patients accepted IBS Symptom Severity Scale (IBS-SSS) score, Self-rating Depression Scale (SDS) and Self-rating Anxiety Scale (SAS) anxiety and depression scores, and also underwent colonoscopy to collect ileum and colonic mucosa specimens. The expression of NMDAR2B in intestinal mucosa was detected by immunofluorescence, and fasting serum was collected to detect the tryptophan, kynurenine (KYN), KYNA and QUIN by high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Our results showed that the KYN pathway of IBS-D patients was activated. The production of QUIN and KYNA was imbalanced and resulting in an increased NMDAR2B for patients with IBS-D, which may be involved in intestinal symptoms and mood disorders of IBS-D. |
format | Online Article Text |
id | pubmed-7388639 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | PAGEPress Publications, Pavia, Italy |
record_format | MEDLINE/PubMed |
spelling | pubmed-73886392020-08-12 Characterization of kynurenine pathway in patients with diarrhea-predominant irritable bowel syndrome Li, Pingping Zheng, Jimin Bai, Yun Wang, Dingxin Cui, Zijin Li, Yueqin Zhang, Jian Wang, Yuzhen Eur J Histochem Article Our objectives are to demonstrate whether the kynurenine pathway is activated in diarrhea-type irritable bowel syndrome (IBS-D) patients, and whether the neurotoxic metabolite quinolinic acid (QUIN) is out of balance with the neuroprotective metabolite kynurenic acid (KYNA), and further explore whether this can lead to increased expression of N-methyl D-aspartate receptor 2B (NMDAR2B) in the enteric nervous system and in turn leads to intestinal symptoms and mood disorders. All enrolled healthy controls and patients accepted IBS Symptom Severity Scale (IBS-SSS) score, Self-rating Depression Scale (SDS) and Self-rating Anxiety Scale (SAS) anxiety and depression scores, and also underwent colonoscopy to collect ileum and colonic mucosa specimens. The expression of NMDAR2B in intestinal mucosa was detected by immunofluorescence, and fasting serum was collected to detect the tryptophan, kynurenine (KYN), KYNA and QUIN by high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Our results showed that the KYN pathway of IBS-D patients was activated. The production of QUIN and KYNA was imbalanced and resulting in an increased NMDAR2B for patients with IBS-D, which may be involved in intestinal symptoms and mood disorders of IBS-D. PAGEPress Publications, Pavia, Italy 2020-06-19 /pmc/articles/PMC7388639/ /pubmed/32705857 http://dx.doi.org/10.4081/ejh.2020.3132 Text en ©Copyright: the Author(s) http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Li, Pingping Zheng, Jimin Bai, Yun Wang, Dingxin Cui, Zijin Li, Yueqin Zhang, Jian Wang, Yuzhen Characterization of kynurenine pathway in patients with diarrhea-predominant irritable bowel syndrome |
title | Characterization of kynurenine pathway in patients with diarrhea-predominant irritable bowel syndrome |
title_full | Characterization of kynurenine pathway in patients with diarrhea-predominant irritable bowel syndrome |
title_fullStr | Characterization of kynurenine pathway in patients with diarrhea-predominant irritable bowel syndrome |
title_full_unstemmed | Characterization of kynurenine pathway in patients with diarrhea-predominant irritable bowel syndrome |
title_short | Characterization of kynurenine pathway in patients with diarrhea-predominant irritable bowel syndrome |
title_sort | characterization of kynurenine pathway in patients with diarrhea-predominant irritable bowel syndrome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388639/ https://www.ncbi.nlm.nih.gov/pubmed/32705857 http://dx.doi.org/10.4081/ejh.2020.3132 |
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