Multiplexed GTPase and GEF biosensor imaging enables network connectivity analysis

Here we generate FRET biosensors for guanine exchange factors (GEFs) by inserting a fluorescent protein pair in a structural “hinge” common to many GEFs. Fluorescent biosensors can map the activation of signaling molecules in space and time, but it has not been possible to quantify how different act...

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Detalles Bibliográficos
Autores principales: Marston, Daniel J., Vilela, Marco, Huh, Jaewon, Ren, Jinqi, Azoitei, Mihai, Glekas, George, Danuser, Gaudenz, Sondek, John, Hahn, Klaus M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388658/
https://www.ncbi.nlm.nih.gov/pubmed/32424303
http://dx.doi.org/10.1038/s41589-020-0542-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388658/
https://www.ncbi.nlm.nih.gov/pubmed/32424303
http://dx.doi.org/10.1038/s41589-020-0542-9

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