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Multiplexed GTPase and GEF biosensor imaging enables network connectivity analysis
Here we generate FRET biosensors for guanine exchange factors (GEFs) by inserting a fluorescent protein pair in a structural “hinge” common to many GEFs. Fluorescent biosensors can map the activation of signaling molecules in space and time, but it has not been possible to quantify how different act...
| Autores principales: | , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388658/ https://www.ncbi.nlm.nih.gov/pubmed/32424303 http://dx.doi.org/10.1038/s41589-020-0542-9 |