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Potential mechanisms of action of celastrol against rheumatoid arthritis: Transcriptomic and proteomic analysis

The clinical efficacy for treating of celastrol rheumatoid arthritis (RA) has been well-documented, but its mechanism of action remains unclear. Here we explored through what proteins and processes celastrol may act in activated fibroblast-like synoviocytes (FLS) from RA patients. Differential expre...

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Autores principales: Xinqiang, Song, Erqin, Dai, Yu, Zhang, Hongtao, Du, Lei, Wang, Ningning, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7390347/
https://www.ncbi.nlm.nih.gov/pubmed/32726313
http://dx.doi.org/10.1371/journal.pone.0233814
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author Xinqiang, Song
Erqin, Dai
Yu, Zhang
Hongtao, Du
Lei, Wang
Ningning, Yang
author_facet Xinqiang, Song
Erqin, Dai
Yu, Zhang
Hongtao, Du
Lei, Wang
Ningning, Yang
author_sort Xinqiang, Song
collection PubMed
description The clinical efficacy for treating of celastrol rheumatoid arthritis (RA) has been well-documented, but its mechanism of action remains unclear. Here we explored through what proteins and processes celastrol may act in activated fibroblast-like synoviocytes (FLS) from RA patients. Differential expression of genes and proteins after celastrol treatment of FLS was examined using RNA sequencing, label-free relatively quantitative proteomics and molecular docking. In this paper, expression of 26,565 genes and 3,372 proteins was analyzed. Celastrol was associated with significant changes in genes that respond to oxidative stress and oxygen levels, as well as genes that stabilize or synthesize components of the extracellular matrix. These results identify several potential mechanisms through which celastrol may inhibit inflammation in RA.
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spelling pubmed-73903472020-08-05 Potential mechanisms of action of celastrol against rheumatoid arthritis: Transcriptomic and proteomic analysis Xinqiang, Song Erqin, Dai Yu, Zhang Hongtao, Du Lei, Wang Ningning, Yang PLoS One Research Article The clinical efficacy for treating of celastrol rheumatoid arthritis (RA) has been well-documented, but its mechanism of action remains unclear. Here we explored through what proteins and processes celastrol may act in activated fibroblast-like synoviocytes (FLS) from RA patients. Differential expression of genes and proteins after celastrol treatment of FLS was examined using RNA sequencing, label-free relatively quantitative proteomics and molecular docking. In this paper, expression of 26,565 genes and 3,372 proteins was analyzed. Celastrol was associated with significant changes in genes that respond to oxidative stress and oxygen levels, as well as genes that stabilize or synthesize components of the extracellular matrix. These results identify several potential mechanisms through which celastrol may inhibit inflammation in RA. Public Library of Science 2020-07-29 /pmc/articles/PMC7390347/ /pubmed/32726313 http://dx.doi.org/10.1371/journal.pone.0233814 Text en © 2020 Xinqiang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Xinqiang, Song
Erqin, Dai
Yu, Zhang
Hongtao, Du
Lei, Wang
Ningning, Yang
Potential mechanisms of action of celastrol against rheumatoid arthritis: Transcriptomic and proteomic analysis
title Potential mechanisms of action of celastrol against rheumatoid arthritis: Transcriptomic and proteomic analysis
title_full Potential mechanisms of action of celastrol against rheumatoid arthritis: Transcriptomic and proteomic analysis
title_fullStr Potential mechanisms of action of celastrol against rheumatoid arthritis: Transcriptomic and proteomic analysis
title_full_unstemmed Potential mechanisms of action of celastrol against rheumatoid arthritis: Transcriptomic and proteomic analysis
title_short Potential mechanisms of action of celastrol against rheumatoid arthritis: Transcriptomic and proteomic analysis
title_sort potential mechanisms of action of celastrol against rheumatoid arthritis: transcriptomic and proteomic analysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7390347/
https://www.ncbi.nlm.nih.gov/pubmed/32726313
http://dx.doi.org/10.1371/journal.pone.0233814
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